Analysis of nuclear area factor after Sorafenib and combination treatment induced apoptosis in DAPI stained HepG2 cells. ImajeJ software was used for calculating circularity 4 pi (area/perimetre2) and roundness (perimeter2)/ (4*pi*area). Nuclear area factor was calculated as the product of object area (in pixels2)*roundness. Data was presented as mean ± SEM and experiments were run in triplicates.