(A) Pathogens (Didymella bryoniae) were validated by conducting the pathogenicity test through (Didymella bryoniae) Koch’s postulates. The pumpkin plants were grown in the pots in the greenhouse. The spore suspension (10°6conidia/ml) was prepared from 7 days old culture of the associated fungus (Didymella bryoniae) and was sprayed on the injured (pin prick method) leaves of 1-2 weeks old spinach plants grown in the pots. Symptoms appeared on the leaves after 7-14 days of inoculation. (B) Plants apparently showed the symptoms of light brown to dark brown spots with concentric rings and leaf margin are curled. These symptoms were compared with the original and pathogen was re-isolated to check the identity and pathogenicity.