Representative PCR-RFLP pattern of IGF-1 gene digested with PstI and separated on 2% agarose gel, L: DNA ladder (100 bp); lane 2, 5, 8, 10: CC genotype (536 bp); lane 1, 3, 4, 6, 11: CT genotype (536/406/130 bp); lane 9: TT (406/130 bp); (-) negative control (PCR product without enzyme treatment).