Characterization of biomarkers on CSPCs. (a) The expression of biomarkers CD90/105/166, Vimentin, and FGFR3 on CSPCs were analyzed by immunofluorescence (bar, 50 μm); (b) RT-PCR was used to detect mRNA expression levels of cell surface antigens of CSPCs; (c) CSPCs were colabeled with biomarkers and analyzed by flow cytometry, and the positive rates of surface antigens of CSPCs were all above 95%.