Phylogenetic trees based on the 16S rRNA sequences of Anaplasma spp. were compared with the neighbor-joining method using the MEGA software (version 11) with maximum likelihood analysis based on the general time-reversible model. Phylogenetic tree showing taxonomic relationships of resulted two sequences; OR654955 and OR654956 (solid red circle) detected from cattle in Yangon region and 14 reference sequences of 16S rRNA of all Anaplasma spp. which can be infected to cattle. The relationships were inferred based on 16S rRNA data, including 1 from dog in Myanmar and 13 reference sequences of other Anaplasma spp. originated from different countries. For each sequence, the accession numbers are followed by full species names, the host and the country of origin.