Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
... field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these s...
Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad
British Journal of Virology
... field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these s...
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
... field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these s...
Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*
...so occurred in different tissues of citrus. Two multiplex molecular detection methods provide the understanding of the genetic diversities among viroid isolates and quantify viroids in citrus host. Our field survey can help clarify citrus-viroid relationships and develop proper prevention strategies in the near future.
...
Suresh V Kuchipudi* and Kin-Chow Chang
...titutes the largest soft tissue mass in the body whose principal roles are postural maintenance and locomotion. Comprising heterogeneous multi-nucleated fibres, skeletal muscle is one of more astounding and unusual tissues in the body in its ability to undergo dramatic phenotypic changes in response to physical demands, age and disease. The contributions of skeletal muscle to immune response and virus pathogenesis are incr...
Hu Zenglei1 and Xiufan Liu1, 2*
..., especially in lymphoid tissues. Genotype VIId of NDV, which is currently endemic in many countries of Asian and Middle East, replicates at a significantly higher level, induces more potent innate antiviral and inflammatory response, and causes more severe damages in lymphoid tissues when compared with virulent viruses of other genotypes. Therefore, severe pathology in immune organs, caused by genotype VIId of NDV, is assoc...
Brittany D. Rife 1,2, Marco Salemi 1,2*
E-mail | salemi@pathology.ufl.edu
...gration between infected tissues and cell types, provides valuable insight into immunopathological processes, and ultimately paves the way for a better understanding of the interplay between viral evolution and pathogenesis....
Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah
Email | mmshah@ciit.net.pk
...;such as genome mapping, tissue culture, genetic transformation and in vitro regeneration offered new dimensions of research to improve important crop species including wheat. The tissue culture by itself has increased the crop improvement potential to develop new cultivars with desirable traits through somaclonal and gametoclonal selection. While successful tissue culture i...
Jonas Johansson Wensman1*a, Karl-Johan Leuchowius2,3 a, Jiting Yan1, Anna-Lena Berg4, Liv Bode5, Hanns Ludwig5, Sandor Belak6, Ulf Landegren2, Ola Soderberg2, Mikael Berg6
...to detect BDV P in brain tissues of infected animals. Finally, protein-protein interactions were visualized in both C6BV and brain tissues of experimentally as well as naturally infected animals (rat and horse, respectively). BDV proteins and their interactions with host proteins could be shown in cell cultures (HMGB1, Cdc2) and in brain tissues of rat (HMGB1, Cdc2) and horse (Cdc2 only) i...
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
...CR in both swab and body tissue samples. The molecular characterization revealed clustering of the PPRV within lineage IV with significant substitutions in the nucleoprotein (NP) gene. Genetic variations within NP gene, and possibly in other proteins which are essentially mediating protective immunity, may explain the extreme infectious nature of the virus and its host-specific pathogenesis. Moreover, understanding the nature of such circulating field viruses ...
Zahir Shah, Shakoor Ahamd, Mian Saeed Sarwar, Murad Ali Khan and Javid Ali
...rotrusion of an organ or tissue through an opening. The present study describes a typical case of hernia in four weeks old cross Holstein Friesian dairy breed calf with hanging mass in the umbilical region. Tentative diagnosis was made through clinical sign and physical examination; the area was incised under local anesthesia. After surgical procedure site was closed by applying mattress sutures. Adjunct therapy of broad-spectrum antibiotic was administered in...
Abdul Qahar and Bashir Ahmad
...lfur content in soil and tissue were substantially increased with application of both the nutrients. However, sulfur content in plant tissue did not vary with increasing N from 250 to 350 kg ha-1, but was considerably improved with respect to control. It is concluded that R-2210 produced maximum yield, BCR value and N content in tissue with 350 kg N ha-1, however S content in soil and in <...
Wan-Long Zhu* and Gao Wenrong
... liver and brown adipose tissue, suggesting that A. chevrieri was more sensitive to cold than that of photoperiod. Together, these data suggested that A. chevrieri mainly depend on increasing thermogenic capacity to cope with cold or winter condition....
Characterization, Tissue-Specific and Developmental Stage Expression of Somatostatin in Coilia nasus
Siyu Yang1, Fukuan Du2 and Pao Xu1,2*
Sania Subhan Qureshi, Mian Saeed Sarwar and Zahir Shah
...ass from the surrounding tissues. Peritoneum, muscle and subcutaneous tissues were sutured with absorbable suture (vicryl; polyglactin 910) and skin with a non-absorbable suture (silk). Owner was advised for daily check-up for any damage to the stitches and for regular dressing of the wound with antiseptic. Antibiotics and analgesic was recommended for 3-5 days respectively. As per feedback of the owner, the wound was healed...
Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*
...ic cell death in splenic tissue of infected mice. Moreover, infection with live P. chabaudi was accompanied with an increase in NO, H2O2, and MDA levels in splenic tissue of lupus mice in comparison to either control or gamma irradiated P. chabaudi- infected group. Our data reveal that infection of lupus mice with live P. chabaudi has several effects on the splenic tissue on the histologic...
Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
...ll 120 blood samples, 24 tissue samples from each organ (trachea, lung and intestine) were collected from the 12 commercial layer flocks selected randomly at the age of 35-50 weeks without any direct clinical manifestation of avian influenza. Serum collected from 120 birds was tested for antibodies against H9N2 by using Haemagglutination Inhibition assay. Calculated geometric mean titers of 5.37 revealed the infectivity of the flock with H9N2 Influenza virus. ...
G.E. Odo1*, E.J. Agwu1, N.I. Ossai2, C.O. Ezea3, J. Madu1 and V. Eneje2
...esults on gill and liver tissues sampled showed concentration and time dependent significant increase (p < 0.005) in the values of malondialdehyde, catalase, superoxide dismutase, reduced glutathione, alkaline phosphatase (ALP), alanine aminotransferase (AST) and aspartate aminotransferase (AST). Similarly, aluminium phosphide enhanced levels of AST, ALT and ALP as both concentration and time dependent in elevation were recorded when compared to the control...
Önder Duysak*andKübra Azdural
...) were determined in tissue of Patella caerulea from various population in Iskenderun Bay, Turkey.Our study focused on seasonal variation of some heavy metals and aluminium in Patella caerulea L., 1758 tissues collected from eight different sampling stations of Iskenderun Bay. The concentration of heavy metal in P. caerulea tissues tended to vary significantly betw...
Faiz Muhammad,1, 2,*, Zhang Zhi-Feng,1, Shao Ming-Yu1 and Muhammad Shafi3
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...phological studies. Bile tissues, aspirates and blood samples of the same animals were analysed. The infection was found prevalent throughout the year but during post monsoon season it was relatively high. Morphological identification of G. expalanatum was carried out on the basis of size and shape of worm. The hematological and bile biochemical changes were found in two groups. Pathologically significant changes were observed in the infected
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2*
... of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked immuno sorbent assay (ELISA) in 2015 and 2016. The diagnosis of avian leukosis virus subgroup A (ALV-A) infection was confirmed by necropsy, histopathological examinations and PCR analysis. The ...
Saqib Ali1, Suliman Ali1, Lina1, Wen Zhou1, Muhammad Irfan Waris1, Ashfaq Ali2 and Man Qun Wang1,*
...e bio-worsening of woody tissue. The females lay eggs on the bark surface of the stems and branches of trees as a result rotten woody plants. The larval beetles characteristically feed in the phloem as well as later in the xylem. The females select living hosts for oviposition and thus destroy the vigour of the trees. However, at the early stage of intestation, detection of Anoplophora glabripennis and exposure will help to eliminate the pest in ...
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
...ted Mycoplasma spp. from tissue samples collected from clinically sick (60 samples) and apparently healthy broiler birds (30 samples) in a commercial farm, using classical isolation and compared with molecular identification of Mycoplasma infections. A total of 96 tissue samples from trachea, air sacs, lungs and oral swabs along with blood from each bird were collected and initially subjected to isolation of Mycoplasma spp. ...
Mustafa Cengiz1*, Jama Hussein Ali2, H. Mehtap Kutlu2, Djanan Vejselova2 and Adnan Ayhanci3
...ive changes in the liver tissue, significant decreased in the number of activated Bcl-2, while increased in the number of Bax and caspase-3-positive hepatocytes, a significantly increase in levels of the activities of biochemistry markers (serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP)). In contrast, in the groups given D-GaIN and EA, a decrease in the damage of the liver tissue...
Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram
Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
...nside the leaf and fruit tissues, respectively. Among them, 70 fungal isolates belonged to plant pathogenic genera like,Alternaria, Cercospora, Colletotrichum, Curvularia, Glomerella, Mycosphaerella, Phoma and Stemphylium and the other 24 isolates of fungi belonged to Aspergillus, Acremonium, Chaetomella,Cunninghamella, Geotrichum, Gliocladium, Monodictys, Mucor, Myrothecium, Penicillium, Periconia and Pithomyces. One-hundred and thirteen isolates of chilli mi...
Goutam Samui and Shantanu Jha
Branch gall of mango (Oligotrophus mangiferae Keiffer) its bioecology and management
...shoots by puncturing the tissue. A full grown larva was yellow in colour with a clear dark brown constriction on head. Initially the pupa was creamy white in colour which gradually turned light yellow and finally blackish. Adults were grey in colour and 2-3mm in length. A single species of parasite (Tetrastichus spp.) was found to emerge from the gall. Pruning at 30 cm had been found to be most effective in managing the pest. Spraying of thiamethoxam @ 0.008%,...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...st formation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...(Pit1)is a member of the tissue-specific POU homeobox transcription factor family that is found in all mammals. Pit1 encodes a pituitary-specific transcription factor, which is involved in pituitary development and regulating the hormone expression in animals. In current study, Pit1 gene was screened for polymorphic sites in Sahiwal cattle. Samples from Sahiwal cattle breeds were sequenced using six set of primers. A total 15 polymorphisms; 12 in ...
Yasemin Bircan Yildirim* and Hediye Tugce Vurmay
...from gills and intestine tissues of commercially important finfish species: saddled seabream (Oblada melanura), gilthead sea bream (Sparus aurata), Bogue (Boops boops), horse mackerel (Trachurus mediterraneus), red mullet (Mullus barbatus), brushtooth lizardfish (Saurida undosquamis), grey mullet (Mugil cephalus), threadfin bream, (Nemipterus randalli), which were caught from the Iskenderun Bay at 6 diffe...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...e established in various tissues, including brain, heart, spleen, kidney, intestine, gill, liver, skin, blood and muscle of common carp. Expression profiles provided us more evidences to understand GST gene functions as well as their functional evolution post duplication. Overall, the whole set of GST genes provide essential genomic resources for future biochemical, toxicological and physiological studies in common carp.
...
Wenqiao Hui1, Jishun Tang1, Dejian Zhu1, Qian Ban2* and Sheng Chen1*
...gical changes in adipose tissue at pre-puberty and puberty onset stages of goats, and then determined the role of leptin to activate signaling pathways which modulate the expression of hypothalamic genes involved in reproduction and metabolism. We found that leptin significantly upregulated the LEPR in the adipose tissue of goat at the puberty onset stage. Moreover, we observed that LEPR, JAK2, STAT3 (P<0.05), KISS1 (P<...
Sogolo L. Lebelo1,* and Gerhard van der Horst2
...bundant loose connective tissue. Leydig cells have large spherical or slightly elongated nuclei, which possess prominent nuclei and frequently deep indentations of nuclear envelope. The nuclei are characterized by patches of euchromatin and heterochromatin regions with the latter usually occupying the periphery of the organelle. The cytoplasm contains numerous mitochondria containing distinct lamellar cristae, small patches of rough endoplasmic reticulum as sh...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and Kui Chen2,*
...l expression spectrum of tissues in combination with bioinformatics analysis so as to understand the possible functions in response to chemical sensation behavior of taste receptors. The receptors when compared with T. rubripes, there was 99.2% identity in two kinds ORF sequences, with only 7 bases different, and there were 5 amino acids different between speculated fusion proteins. Under bioinformatics analysis, the T2R1 gene without introns was...
Zakirullah Jan1, Shamsher Ali1*, Tariq Sultan2, Wasiullah1 and Wiqar Ahmad1
...ters of rice crop, plant tissue and post-harvest soil analysis. But in this paper, data on availability of major plant nutrients, soil organic matter and plant dry weight are presented. The data revealed that cyanobacteria strains had significantly (p < 0.05) increased the availability of major plant nutrients and SOM under the saline soil conditions. Among the strains, Gloeobacter MMF-4 significantly increased the soil organic matter (SOM), nitrogen (N), p...
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
...l tumor and 100% in soft tissue sarcoma) and feline (60% in mammary adenocarcinoma and 100% in soft tissue sarcoma) tumors studied. These findings might support the established role of c-Myc in tumor pathogenesis but how this transcription factor affects the downstream targets to achieve the goal remains unanswered. Therefore, further research is desperately needed to understand the mechanism of tumor pathogenesis in animals...
Xue-yang Wang1, Shang-zhi Zhang1, Ming-hui Liu2, Dong Yu1, Yan Ma1, Dong-qiong Fei1, Hai-zhong Yu1 and Jia-ping Xu1,*
...rescence in all analyzed tissues, including head, midgut, hemolymph, fat body, testis and ovary. More importantly, the relatively high expression level of BmARM-like mRNA were observed in BC9 (near-isogenic line) following BmNPV infection as compared to P50 (susceptible strain), which was further validated in the midgut of A35 (resistant strain). The expression levels of BmARM-like protein showed similar trends with transcriptional levels in the midgut ...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...ity for several hours in tissue samples. For this study piglets without colostrum feeding were selected. Primary cell culture and subculture was performed from the intestinal epithelial cells of piglets. Then the effects of intestinal trefoil factor (TFF3) in intestinal disease was explored. Porcine intestinal epithelial cells were treated with lipopolysaccharide (LPS) and recombinant human (rh) TFF3. Cell proliferation and TFF3 mRNA expression were examined b...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
...n analysis in five camel tissues was examined employing real-time PCR. The highest level of transcripts was found in the camel testis, followed by liver, spleen, kidney and lung. CdGSTP1-1 was heterologously expressed in Eschericia coli BL21(DE3) as a ~24 kDa soluble protein and showed to be catalyticly active towards the model substrate 1-chloro-2,4-dinitrobenzene. The results of the present study provide new information into camelid evolution a...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
...ng the growth of mammary tissue during pregnancy and its release during pregnancy accounts for increased mammogenesis. Bubaline placenta was obtained and processed for extraction of extracellular proteins at pH 9.5 using Tris-Glycine buffer. Placental lactogen was purified by gel filtration chromatography using Sephadex-G100 and ion exchange chromatography on DEAE- Sepharose, employing a linear salt gradient. SDS-PAGE was used to monitor the purification steps...
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
...cated in neutrophils and tissue that likely plays a vital role in host defense against GNB (gram-negative bacteria) and their endotoxin by means of its antibiotic and endotoxin neutralizing and disposing functions. BPI has several biological functions in mammals such as human, bovine, pig and rabbit, it also has major influence in the natural defense of the animal body. In this paper, by comparative study on BPI gene expression in various
LX Ye1,2, JX Wang1,2,*, P Li1,2 and XT Zhang1,2
...rtments and interstitial tissue. The tubular compartments were comprised of the seminiferous tubules, straight seminiferous tubules and rete testis, without any distinct boundary tissue between them. The interstitial tissue between the tubules contained many Leydig cells, tiny veins and connective tissue. Ghrelin-ip cells in the African ostrich testes we...
H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2
... respectively. Different tissues specimens of 104 confirmed seropositive cows under investigation including, retropharyngeal, prescapular, prefemoral, internal iliac, supramammary lymph nodes, udder and spleen as well as milk of 46 lactating cows were subjected for bacteriological studies for isolation and identification of Brucella organisms. Brucella melitensis biovar 3 could be recovered from 64 (61.5%) tissue specimens a...
Serap Gelibolu1,*, Yasemen Yanar2, M. Ayce Genc3 and Ercument Genc4
...nce, body physiology and tissue morphology of gilthead seabream (Sparus aurata). Treatment of fish with MOS-feed shown a significant increase in live weight and protein efficiency rates when were directly compared with mock-treated fish control. However, there was no statistically supported level of significance was observed for growth rates and feed conversion rates among groups. Improved live weight and protein efficiency rates reflected positively on...
Xiaoting Zhang1,2, Jiaxiang Wang1,2,*, Peng Li1,2,* and Lixun Ye1,2
...composed of interstitial tissue and parenchyma. The interstitial tissue formed a layer of serosa on the surface of the lung. The parenchyma of the lung consisted of bronchial levels, atria and respiratory capillaries. The bronchi were divided into the primary, secondary and tertiary bronchi. The wall of the tertiary bronchus had openings for the atria and respiratory capillaries. The respiratory capillaries were surrounded b...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...in different bull testes tissues (1-day, 12-months-old and 24-months-old). In addition, genes mRNA and protein levels were detected after bull testicular Sertoli cells were transfected with miR-122 and miR-449a. Meanwhile, DUSP4, PDK4 and FKBP1B overexpression experiments were conducted. Eventually, MTT assay was performed to observe the cells proliferation. The results showed that miR-122 and miR-449a were highly-expressed in testis
Soraya Khosravian Dehordi*, Abdolnaser Mohebbi and Kahin Shahanipour
... were reduced in splenic tissue of Cd-intoxicated rats, but the nano-Se was able improve these undesirable effects. However, these findings imply that the nano-Se could be an efficient material in cases of Cd-intoxication....
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
Abd El-Nasser Ahmed Mohammed*
...ansplantation of ovarian tissue has been shown to induce puberty and pregnancies successfully. The aims of the present study were to evaluate ovaries age effect on restoration function upon transplantation in mice and rats. Eighteen adult female rats were classified to three equal groups in addition to sex young females as donor of young ovaries. Similar classification was done using eighteen female mice and sex young females. The females of mice or rats were ...
Tania A. Shakoori1,*, Soumble Zulfiqar2, Khadija Irfan Khawaja3 and Faisal Masud4
...Akt, and mTOR in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed decreased genetic expression of AMPK (0.04 fold, p<0.001), mTOR (0.30 fold, p=0.04), Akt (0.29 ...
Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar1
...a particular endometrial tissue exterior to the uterus. It is a principal cause of long lasting morbidity, mostly from serious pelvic pain(1). Endometriotic tissue has the potential for aromatase gene expression that leads to aromatase and estrogen production. Danazol is a synthetic androgen whereas Letrozole is aromatase inhibitor. This study was conducted to compare the mean decrease in visual analogue scale (VAS) pain sco...
Mian Sajjad Ahmad1, Somia Iqtadar1, Sami Ullah Mumtaz1*, Zafar Niaz1, Iqra Waheed2 and Sajid Abaidullah1
...e of excess body adipose tissue. Impaired glucose tolerance is a risk factor for developing diabetes Mellitus & is associated with hyperglycemia and insulin resistance. To determine the frequency of impaired glucose tolerance in obese patients. This Cross Sectional study was carried out in North Medical Ward, King Edward Medical University/ Mayo Hospital Lahore for 6 months i.e. January to June 2014. Total 160 obese patients visiting outpatient department ...
Nay Naing Htoo1, Basit Zeshan1,2,*, Aung Tun Khaing1, Than Kyaw1, Erkihun Aklilu Woldegiorgis1 and Mohd Azam Khan1
...than DM group. The rumen tissue weight of creep-fed kids was 17% greater than those of milk fed kids. Overall, the present study showed that the creep feed supplementation to nursing goat kids improve rumen morphology.
...
Nazish Shaukat1, Muhammad Javed1,Faiza Ambreen2* and Fariha Latif1
...hich ultimately leads to tissue damage and oxidation of biomolecules. Aquatic organisms possess antioxidant system to cope with the oxidative stress. This research was conducted to see the effect of lead on peroxidase activity (antioxidant enzyme) in the gills and liver of Labeo rohita. Experiment was conducted in the laboratory by exposing four groups of Labeo rohita to different doses of PbCl2 viz. 96-h, 2/3rd, 1/4<...
Arshad Ali, Imdad Ali Mahmood, Muhammad Salim, Muhammad Arshadullah* and Abdul Rasool Naseem**
GROWTH AND YIELD OF DIFFERENT BRASSICA GENOTYPES UNDER SALINE SODIC CONDITIONS
...c concentration in plant tissues and oil content in seeds were also determined. Comparatively more number of branches and pods per plant were produced by cultivar Dunkled closely followed by BARD-I while maximum seed yield (241.7 and -1 235.1 kg ha ) was obtained from Dunkled and Sultan Raya, respectively which was statistically at par. However, BRS-II and Rainbow showed significantly more percent oil contents in their seeds but genotype Dunkled showed minimum...
Nazakat Nawaz*, Malik Shah Nawaz*, Mubashir Ahmad Khan*, Mirza M. Yasin*, Doulat Baig*, Nasir Mehmood Cheema**, Muhammad Amjad** and M. Altaf Sher**
EFFECT OF BORON ON PEANUT GENOTYPES UNDER RAINFED CONDITIONS
...tions in different plant tissues were higher in all genotypes as compared to control treatments during the two years. The boron using efficiency of peanut genotypes declined in the order of BARD-479 > ICG- 7326 > ICGV-92023. A boron fertilizer requirement observed for the -1 highest dry pods yields was 1.0 kg B ha for all genotypes and yield decreases gradually after certain level by increasing boron fertilizer rates....
Muhammad Ali*, Ghulam Mustafa Sajid**, M. Faisal Anwar Malik*** and Kalimullah****
ESTABLISHMENT OF IN VITRO CULTURE OF GRAPES
...p explants (meristemetic tissue) of grapes was investigated through tissue culture technique. These explants were collected from gene bank of Institute of Agricultural Biotechnology and Genetic Resources (IABGR), National Agricultural Research Centre (NARC), Islamabad, Pakistan. Fifteen accessions of grapes were surface sterilized and tested on 75% MS media for germination and initial growth parameters. Accession No. 020017 ...
Youbao Zhong1, Xianlai Zhang1, Xiaofen Hu1 and Yong Li1, 2,*
...he crypt. The connective tissue was denser in the jejunal submucosa, but the muscularis was thicker. The intestinal glands were greatly developed in the ileum, lymphoid nodules were observed in the mucosa, and diffuse lymphoid tissue was distributed in the lamina propria. Meanwhile, goblet cells gradually increased from the duodenum to the ileum. The mast cells showed various shapes and sizes in the small intestine and could...
Hao Zhang,1,2,3,* Hai Tao Nie,3 Tie Wei Ma,3 Zi Yu Wang3 and Feng Wang3
...butions in the main body tissues and the net requirements for maintenance and growth of F1Dorper × Hu ewe lambs. Thirty-five ewe lambs averaging 33.52±0.56 kg body weight (BW) were used. Seven ewe lambs were randomly chosen and slaughtered at 34.93 ± 0.37 kg BW as the baseline group for measuring initial body composition. Another seven lambs were also randomly chosen and offered a pelleted mixed diet (approximately concentrate : ...
Hongwei Liang1,2,*, Miaomiao Tong1, Lihuan Cao1,2, Xiang Li3, Zhong Li1 and Guiwei Zou1,*
...cids than muscle. Muscle tissue of the Huaihe River strain had the highest concentration of pharmacodynamic amino acids and the highest concentration (P < 0.05) of arachidonic acid (females: 109.83 mg/ 100g, males: 110.72 mg/100 g). Concentrations of eicosapentaenoic acid and docosahexaenoic were higher in male muscle than in female muscle. Opposite results were obtained with calipash. The n-3-to-n-6 polyunsaturated fatty acid ratios were within the ...
Shagufta Andleeb*1, Shabana Shaukat1, Chaman Ara2
...rough bioaccumulation in tissues. Aim of present study was to analyze various developmental abnormalities by a sub-lethal dose of cadmium chloride and protective role of garlic (Allium sativum), to minimize the intensity of these toxicities. For this purpose, fertilized eggs of Gallus domesticus were randomly divided into four groups of forty eggs each. Control group was intact and untreated. Eggs of one group were injected with a sub-lethal dose...
Song Jiang1,2, Fa-lin Zhou3, Jian-hua Huang1, Qi-bin Yang1, Li-shi Yang1 and Shi-gui Jiang1,*
..., source of energy, with tissues and organs also important energy suppliers. The results of the present study will hopefully improve our understanding on the characteristics of feeding, gonad development, and energy conversion of P. monodon broodstock during their breeding season, and provide a theoretical foundation for the scientific cultivation of P. monodon broodstock.
...
Sherin Reda Rouby
...R) (RPO30) together with tissue culture-adapted cattle LSDV/Ismailyia88 strain and two Sheep poxvirus vaccinal strains as control. LSDV yield amplicon differed in length by 21 nucleotides from those produced from SPPV either field or vaccinal strain. Among different LSD clinical samples, skin nodules and scabs are proved excellent sample material as they yield clear DNA bands that reflect the high concentrations of the virus. The results of the current study c...
Leila A. Kaimbayeva1,*, Elena S. Malysheva2, Rashit Kazikhanov3 and Saule R. Kazikhanova4
...ing power, activities of tissue enzymes and microstructure changes. The results of chemical analysis revealed the nature of protein substances, altered рН value of the meat and the intensity of glycolysis. All these parameters were indicative of autolysis process in the meat. The levels of water-binding capacity and structural-mechanical properties of red deer meat that occurred during the course of autolysis were further confirmed by the histologi...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...akistan. Altogether, 507 tissue and swab samples were screened for the presence of viral RNA by reverse transcriptase polymerase chain reaction (RT-PCR). Same samples were further processed for the isolation of virus by embryonated egg inoculation technique. Moreover, out of 507 samples, 479 serum samples were scrutinized by enzyme linked immunosorbent essay (ELISA). Sero-prevalence of AIV among different species of wild birds was as follows; peacock (n=35; 14...
Zakirullah Jan1, Shamsher Ali1*, Tariq Sultan2, Muhammad Jamal Khan1, Zahir Shah1 and Farmanullah Khan1
...nd rice shoots and roots tissue analysis for various nutrients uptake/concentration. The data revealed that all strains of cyanobacteria had significantly (p < 0.05) improved the nutrients up-take/concentration and agronomic traits of rice crop as compared to control. Among the strains, the Gloeobacter strain (MMF-4) surpassed by augmenting all agronomic parameters of rice crop like plant height (85 cm), plant fresh weight (45 g), dry weight (24.5 g) and ro...
Mohammed Al Bratty1, Hassan A. Alhazmi1, Somaya Jadoh Ogdi1, Jana Ahmad Otaif1, Abdul Jabbar Al-Rajab2, Mohammad Firoz Alam3 andSadique Akhtar Javed1,*
...g the least contaminated tissues. Importantly, the lead (Pb) content was found to be of serious concern in the tested chicken samples, as it was measured to be higher than its maximum permissible limit (0.1 mg/kg) in poultry meat set by WHO and European Union. The estimated daily intakes (EDIs) of the tested metals through the consumption of the Baladi chicken were found to be lower than their respective reference oral doses (RfD) set by USEPA. The non-carcino...
Tengfei Lu1, Wenhua Pei1, Shuang Zhang2, Yangnan Wu1, Fenghao Chen2, Xiao Han2 and Weijun Guan1,*
...a prospective source for tissue engineering. Amniotic mesenchymal stem cells (AMSCs) obtained from amnion do not evoke ethical issues, as they do with the embryonic stem cells. However, a good many experimental tissues and organs were obtained from model animals but rarely from livestock. In this study, AMSCs were obtained from aborted sheep fetuses (1-3 month) under sterile conditions. Primary AMSCs were sub-cultured to pas...
Tayfun Karataş
...rves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss). Eight-days of fasting caused a significant decrease in glucose, total protein, triglyceride, cholesterol, high-density lipoprotein and low-density lipoprotein levels as well as protein and lipid reserves in liver and muscle tissue of fish (p<0.05). The fasting period had no significant effect on the hepatic thiobarbituric acid reactive substan...
Selçuk Kaplan
...hly expressed in adipose tissue. Leptin is one of the most significant candidate gene marker for MAS studies. Therefore, bubaline leptin gene exon 2, part of the intron 1 and intron 2 region were amplified and sequenced to identify nucleotide variations in Anatolian water buffaloes. Sequence analysis were revealed seven polymorphic site (G1072A, T1081C, T1131G, T1143C, T1145G, T1151G and C1221T) and one monomorphic site (C1071T) in Anatolian water buffaloes. T...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
... accumulation of adipose tissue is largely influenced by the genetic background of cattle. Therefore, we analyzed the transcriptome changes within adipose tissue in beef cattle at different stages of growth. Microarray analysis of data at 12 vs 18 months, 12 vs 24 months, 12 vs 30 months, 18 vs 24 months, 18 vs 30 months and 24 vs 30 months uncovered a total of 10625 differentially expressed genes (P ≤ 0.05; no fold chang...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...d to investigate whether tissue plasminogen activator (tPA) could be applied as signal peptide for enhancing the effect of somatostatin DNA vaccine. A novel recombinant plasmid pEGS/2SS-tPA encoding two copies of somatostatin genes fused to tPA signal sequence was succesfully constructed and identified in MCF-7 cells. Subsequently, thirty female mice randomly were divided into three groups, treating with 50 μg pEGS/2SS-tPA (group T1), 50 μg...
Hanif Ur Rahman1, Umer Saddique1, Zahoor-ul-Hassan1, Shakoor Ahmad1, Muhammad Kamal Shah1, Said Sajjad Ali Shah1, Farhan Anwar Khan1, Fazli Rabbani2, Muhammad Asif Hussain2, Attaur-Rahman2, Irshad Ahmad3,4 and Sadeeq ur Rahman2,*
..., pleural fluid and lung tissues, and streaked into Pleuropneumonia like organism (PPLO) medium for isolation and identification of Mycoplasma species. Confirmed mycoplasma isolates were subjected to species specific PCR to identify members of MM cluster. Results revealed that 79/300 (26.3%) samples were found positive for the growth of Mycoplasma. MM cluster specific PCR showed 49 (16.3%) samples were found positive, of which 34 (11.3%) were fou...
Rafa Almeer1,*, A. Alqarni1,2, S. Alqattan3, S. Abdi4,*, S. Alarifi1, Z.Hassan5 and A. Semlali6
...gene expression of three tissue inhibitors of metalloproteinase (TIMPs) and two matrix metalloproteinase (MMPs) were measured by real-time PCR. All subgroups exhibited altered morphology with accelerated detachment compared to untreated cells. The MTT assay after 48 h revealed that treatment with H1 and H2, reduced cell viability by 48% and 91% respectively, compared to that of untreated cells. These results suggest that anticancer effect of Sidr and Wild hone...
Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
...es 64, hair 13, blood 5, tissues 4) from 5 geographic langur populations of Pakistan and Azad Jammu and Kashmir (AJK) and succeeded in extraction of DNA from 23 samples, which were used for further genetic analysis. RAPD makers (n=8) produced 245 bands (30.62±2.87 Mean±SE / primer) of different molecular weights (126-3342 bp), of which, 96 were population specific. Polymorphism was (37.71±5.29%; mean ± SE), with the highest in Muzaf...
Binbin Shan, Yan Liu, Changping Yang, Shengnan Liu and Dianrong Sun*
...s from four combined tissues (eyestalk, muscle, intestinal and viscus) using High-seq sequencing technology. A total of 65,703,216 high-quality clean reads were generated to produce 27,850 non-redundant transcripts with a mean length of 918 nt using Trinity and Tgicl software. For homological alignment, 13,083 unigenes had significant hits in Nr database. And then, 10,467 unigenes were annotated into three ontologies: biological processes, cellular compone...
Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
... well as colonic mucosal tissue TLR4/9 and NF-kB. We got the following results: (1) The rat IBS-D model was successfully prepared; (2) Wumei Pill could reduce the contents of serum TNF-α, IL-6 and LPS in rats(P<0.05), and alleviate the inflammatory reaction and accumulation of bacterial endotoxins; (3) Wumei Pill could reduce the transcriptional level of TLR4/9mRNA, to decrease TLR4/9 protein synthesis and expression. So we concluded that Wumei Pill p...
Hafiz Muhmmad Tahir1*, Rabia Yaqoob2
Comparing the efficiency of Taq DNA polymerase and PuRe Taq Ready-To-Go PCR beads in amplifying 12S and 16S ribosomal genes
...and up to five years old tissue samples, were compared. Taq DNA polymerase was found to be less efficient compared to the PuRe Taq Ready-To-Go PCR beads for amplification of the 12S rDNA gene. However, difference in the efficiency of both procedures was not statistically significant for the 16S rDNA gene. Furthermore, Taq DNA polymerase was found only efficient for fresh samples while PuRe Taq Ready-To-Go PCR beads were equally efficient for both old and new <...
Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2
Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...erations in reno-hepatic tissue by free radicals scavenging and metal chelating abilities due to presence of anthocyanin, flavonoids and β-sitosterol. The JFE’sprotective effects against heavy metals environmental toxicants especially Cr+6 is novel and cheapest; that should be sponsored for ethno-medicinal purpose....
Asma Karim1, Wajid Ali1*, Noreen Ahmad1, Muhammad Irfan2, Hafiz Abdullah Shakir3
Histological and biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin)
...ure. The blood and liver tissue sampling was done after 96hrs for histology and
biochemical analysis [alanine amino transferase (ALT), and aspartate amino-transferase (AST)]. Liver histology
revealed that necrosis, nuclear pycnosis, hypertrophy of hepatocytes, vacuolization, nuclear atrophy and congestion
of blood vessels were observed as compare to control group. This result was also supported by the significant
increase in levels ...
biochemical analysis [alanine amino transferase (ALT), and aspartate amino-transferase (AST)]. Liver histology
revealed that necrosis, nuclear pycnosis, hypertrophy of hepatocytes, vacuolization, nuclear atrophy and congestion
of blood vessels were observed as compare to control group. This result was also supported by the significant
increase in levels ...
Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1
Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
...d to evaluate changes in tissue and serum total proteins and
albumin along with urea, uric acid and serum transaminases activity after 12 and 24h of TAA administration in albino
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume of normal saline solution...
albumin along with urea, uric acid and serum transaminases activity after 12 and 24h of TAA administration in albino
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume of normal saline solution...
Khurram Shahzad1*, Muhammad Naeem Khan1, Farhat Jabeen2, Nasreen Kosour3, Muhammad Sohail1, Muhammad Khalil Ahmad Khan1, Munir Ahmad1
Bioaccumulation of manufactured titanium dioxide
...eeze dried. Freeze dried tissue samples
were digested with the help of nitric acid (HNO3) and perchloric acid (HClO4). Acid
digested samples were analyzed by Inductively Coupled Plasma Mass
Spectrophotometry (ICP-MS) foraccumulation of Ti, Cu and Zn nanoparticles in
various tissues (gills, liver and muscles). Ti and Cu accumulation in gills was
recorded to be 5.7033 and 3.7133 μg/Kg resp...
were digested with the help of nitric acid (HNO3) and perchloric acid (HClO4). Acid
digested samples were analyzed by Inductively Coupled Plasma Mass
Spectrophotometry (ICP-MS) foraccumulation of Ti, Cu and Zn nanoparticles in
various tissues (gills, liver and muscles). Ti and Cu accumulation in gills was
recorded to be 5.7033 and 3.7133 μg/Kg resp...
Shagufta Nighat1, Muhammad Sajid Nadeem1,*, Syed Israr Shah1, Amber Khalid1, Tariq Mahmood2, Ayesha Aihetasham3 and Muhammad Asif4
...n) concentrations in bat tissues through atomic absorption spectrophotometer. Our findings showed that metals were more concentrated in the liver and kidneys, Zn and Pb showed high mean values as compared to other metals but these values were below the lethal levels. Within the regions and sexes no significant variations were found in metal concentrations; however within three bat species metal concentrations varied significantly. This study provides baseline ...
Ghulam Ali1,3, Wopke van der Werf2 and Just M. Vlak1,
...in dissolution of larval tissues and accumulation of viral occlusion bodies) exhibited reduced food intake and weight gain relative to uninfected larvae. The unexposed larvae and the larvae that were exposed but survived exhibited the same food consumption and weight gain. This study did thus not reveal any sub-lethal effects of exposure to the virus on food consumption and weight gain. There was no viral dose dependency observed in food intake or weight gain ...
A.H. Shahzad1, A. Sattar1,*, A. Husnain1, I. Ahmad2, N. Ahmad1, D. Nak3 and Y. Nak3
...sectional area of luteal tissue was measured on d30 in pregnant animals in both groups. In experiment II, both control (n=64) and resynch (n=54) groups were similarly treated as in experiment I, without P4 profile and luteal cross-sectional area measurement. Pregnancy rate(PR) was compared by PROC FREQ of SAS. Effect of treatment on circulatory P4 profile and luteal tissue cross-sectional area were analyzed usi...
Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*
...n cause defect of dental tissue, mainly attributed to Streptococcus mutans, Actinomyces viscosus, Lactobacillus and Streptococcus sanguis. Capsaicin extracted from chili peppers were selected to explore the antibacterial activity against the four cariogenic bacteria in terms of acid producing and biofilm. The antibacterial activity of capsaicin was evaluated by the minimal bactericidal concentration, change of pH in the first 12 h a...
Fariha Latif* and Muhammad Javed
...significantly in all the tissues as compared to control. Catalase activity varied significantly at p<0.05 among C. catla, L. rohita and C. mrigala. During present research the maximum catalase activity was observed in the liver followed by gills, kidney, brain and muscles. As a conclusion, the sensitivity, bioaccumulation and antioxidant capacities of different fish species based on their ecological and physiological differences may be useful ...
Hua-Lun Luo, Yi-Yu Zhang*, Yuan-Yu Qin and Lei Wu
...xpressed in all examined tissues. ChREBP mRNA level was the highest in abdominal fat and the lowest in gizzard. The g.247075G>A silent mutation in exon 10 was first identified by direct sequencing approach, and resulted in three genotypes of AA, GA and GG. Association analysis demonstrated that the liver ChREBP mRNA expression level was significantly positive or negative effect on serum total protein (TP), albumin (Alb), triglyceride (TG), total cholesterol...
Iqra Bano1,*, Moolchand Malhi2, Pershotam Khatri3, Saeed Ahmed Soomro2, Hira Sajjad4, Ambreen Leghari5, Muhammad Awais2, Safia Kandhro6 and Shakeel Ahmed Lakho5 and Munaza Soomro7
...D activity in testicular tissues was also significantly increased in SY as compared to C. The results revealed that the SY supplementation has positive effects on gross morphological parameters of testis by increasing its weight, thickness, and circumference. Besides this, the SY also improved the height of germinal epithelium of Seminiferous tubule and improved antioxidant status by enhancing the activities of GPx and T-SOD in both serum and testicular
Luo Lei1, Xingxing Deng1, Dengyue Yuan2, Zonglin Zheng1, Chengke Zhu1, Hui Luo1, Baohai Li3, Hua Ye1,* and Chaowei Zhou1,3,*
... mRNA distribution in 21 tissues and investigated the effect of its expression at different nutritional levels. Herein, we found that gibel carp npy has an Open Reading Frame (ORF) containing 291 bp. Moreover, the expression of npy was detected in all tested tissues, with the hypothalamus showing the highest expression in gibel carp. The npy mRNA expression in the hypothalamus significantly decreased 1 a...
Zafrullah Khan* and Shah Alam Khan
...otato, feeding on phloem tissues of leaves of potato plant and significantly reduces the yield of potato. Antixenosis experiments were performed to assess the resistance potential of eight different potato cultivars against M. persicae under glasshouse conditions for three different durations of time (12, 24 and 48 hours) during spring and autumn potato growing seasons of 2016. Both the experiments were conducted using a completely randomized design. Each expe...
Khadim Hussain Wagan1*, Muhammad Ibrahim Khaskheli2, Jamal-U-Ddin Hajano1 and Abdul Ghani Lanjar2
...rowing season. The plant tissues were carrying more M. phaseolina cfu as compared to soil samples. However, cfu count of M. phaseolina was significantly varying among districts, locations and year of samplings (Df= 54, F= 4, P= 0.0000). Similarly, as soil samples collected from Badin, plant tissue samples also gave maximum cfu per gram of M. phaseolina from Badin and then reduced gradually in Thatta, Tando M. Khan, Hyderabad...
Saleha Gul1,2, Muhammad Khisroon2, Ajmal Khan2, Attaullah1, Saira Gul3 and Gul Nabi Khan1,4,*
...cigarette smoke on other tissues are largely unknown. We explored the genotoxic effects of tobacco smoke on peripheral lymphocytes of active and passive smokers. Blood samples were isolated from 100 males’ including 25 active-, 25 passive- and 50 non-smokers. Alkaline comet assay was done and classes were defined on the basis of comet tail length. Significant differences were found in total comet score (TCS) among different groups (p˂0.05). The T...
Şaban Çelebi
... heart, breast and thigh tissues of laying hens were determined in present study. A total of 96 White Lohman laying hens, aged 24 weeks, were randomly divided into 4 groups (n=24), each of which was composed of 6 subgroups. The control group received the basal diet (T-1), treatment groups were fed on the the basal diet plus the three experimental diets included one of the followings: 125 mg/kg vitamin E + basal diet (T-2); 0.5 mg/kg Selenium + basal diet (T-3)...
Jie Yang
...s expressed in different tissues. It is significant to make a study of human PGLYRP1 because neutrophils are a more dominant mechanism in human host defense. Related research results show the functions of human PGLYRP1 in the innate immunity of neutrophils is to conducive to the killing of intracellular and extracellular bacteria. Bactericidal activity of human PGLYRP-1, PGLYRP-3, PGLYRP-4, and PGLYRP-3:4 for both Gram-positive and Gram-negative bacteria requi...
Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*
...ion. The skeletal muscle tissue were studied from fetal sheep, and the stepwise digestion by XI collagenase and trypsin was used to isolate MDSCs and were purified by differential attachment technique. Cell morphology were observed using an inverted microscope, and MDSCs proliferation pattern were determined through growth curve analyses. MDSCs were identified by immunofluorescence and RT-PCR, and the immunofluorescence antibody invoved Sca-1, CD34, CD144, Des...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...haride polymers of plant tissues into simpler monomer like D-galacturonic acids. In this study, polygalacturonase from grape skin was purified by salting out with ammonium sulfate, gel filtration on Sephadex G-75 column and ion exchange on Q-Sepharose column chromatography. Polygalacturonase was recognised as a protein with 47kDa molecular weight by SDS-PAGE. The optimum pH of purified polygalacturonase activity was found to be 4.5 and stable within pH range 3...
Muhammad Usman Ghani1*, Muti Ur Rehaman Khan1, Asim Aslam1, Zubair Shabbir2, Li Bo3 and Naveed Anwar4
Shengjie Zhou1,2, Pengfei Wang1,2, Chao Zhao1,2, Mingjun Fu3, Jian G. Qin4, Lihua Qiu1, Zhenhua Ma1, 4,* and Maoshang Lin1
... showed that the highest tissue expression of L-FABP gene was observed in fish liver on 18 DPH. Along with the ontogeny of fish larvae, the expression of L-FABP gene was low at hatching, and quickly increased with the increase of fish age from 0 DPH to 4 DPH, and reached the highest level on 12 DPH. The highest expression of L-FABP gene was observed in fish cultured at 29°C on both 12 DPH and 18 DPH (P< 0.05). Nutrition enhan...
Sher Bahadar Khan1,2,*, Zou Geng1, Cheng Yu-ting1, Asad Sultan3, Mumtaz Ali4,5, Irshad Ahmad6 and Rui Zhou1
...y pigs from 5 farms), 80 tissue samples from different slaughter houses (20 each intestine, liver, meat and kidney), and 80 samples from super- and wet-markets (each 20 meat and liver) collected both in summer and winter. Isolates were tested for 15 antibiotics (CRO, AMX, GEN, STR, TET, CHL, CLR, LVX, OFX, GAT, CIP, SXT, AMP, LIN and AZM) according to the disc diffusion method and antibiotic resistant genes {tet(A), tet(B), tet(C), strA/strB, aadA, aac(3)IV), ...
Pin Lyu1, Xiangxian Chen2* andQinlong Liu3*
...ds. The injured muscular tissues and blood samples were collected pre-injury and post-injury on day 1, 2, 4, 6, 9, 12 and 15. The exercise and massage group showed the fastest recovery reflected by muscular histological structure and C-reactive protein levels, followed by the other three groups. C-reactive protein decrease in exercise and massage, massage only or exercise only groups was significantly different comparing to control group (p<0.05).Com...
Hong Zhang1, Shu-Fen Han2, Jing Wang1, Shao-Kang Wang1, Gui-Ju Sun1 and Cheng-Kai Zhai1, *
...xpression in rats’ tissues. Compound whole-grain ameliorates diet-induced obesity and hyperlipidemia by enhancing PPARγ and reducing SREBP-1c in rats.
...
Bo Zhang1, Changbo Zhu1, Zihao Meng1,4, Baosuo Liu1, Lian Zhong3, Guiju Huang1, Jiaqi Su1, Sigang Fan1 and Dahui Yu1,2,*
...ectively. Generally, the tissue-specific carotenoid distribution is ranked in the order being adductor < mantle < gill < visceral mass. Significant correlations were found between TCC and the color parameters, with the highest fit (r = 0.908) in the Pearson’s correlation between the color parameter, a* (red degree), and TCC of the adductor. Measuring the a* is, therefore, likely to be an appropriate, rapid, reliable, and nondestruc...
Shakeel Ahmad Anjum1, Sami Ullah1*, Muhammad Mohsin Raza2, Mohsin Raza1, Muhammad Abbas3, Ijaz Ahmad4, Malik Muhammad Yousaf2, Muhammad Zeshan5, Adeel Abbas1, Mehmood Ali Noor1 and Mohsin Nawaz1
... relations, meristematic tissue growth, carbohydrate metabolism, and pollen tube growth which suggests plausible effects on all these activities due to its deficiency. Therefore, a field trial was set up to assess the efficacy of different levels (control, 0.01 M and 0.05 M) of foliar applied B at booting, anthesis, and grain filling growth stages of wheat crop respectively. Results revealed that B application at the rate of 0.05 M solution substantially incre...
Imdad Ali Mahmood1, Muhammad Imran2, Muhammad Sarwar1, Matiullah Khan1, Muhammad Aqeel Sarwar2, Shoaib Ahmed1 and Shahid Riaz Malik2
...n concentration in plant tissues and grains. A significant increase in pods per peduncle, seeds per pod, grain yield and total biomass of lentil was observed with Zn application even at lower rate (5 kg ha-1) under PGPR inoculation. Maximum 100-seed weight (2.56 g) was recorded with PGPR inoculation which was 21 % higher than that of without PGPR inoculation. Overall, a significant increase (31 %) in grain yield and total biomass was observed due to Zn applica...
Muhammad Yasin1, Romana Shahzadi1, Muhammad Riaz2, Mahideen Afridi2, Wajya Ajmal3, Obaid Ur Rehman2, Nazia Rehman3, Ghulam Muhammad Ali1,3, Muhammad Ramzan Khan1,2,3*
...o expression in the leaf tissues was observed. A strong expression for FUL gene was detectable in mature silique and silique from upper portion of plant as compared to other tissues of “Pakola” and “Punjab Sarsoon 3”. Our results flaunt basic gene expression information about shattering genes for developing genome edited plants to prevent yield losses in canola in future. ...
Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3
...8. Sixteen pooled bursal tissue samples were collected from IBD suspected (9 Egyptian balady and 7 broiler) chicken flocks located in different localities at Sharkia governorate, Egypt. These samples were subjected for direct detection of a 620 bp hypervariable region in the VP2 gene using Reverse transcriptase polymerase chain reaction (RT-PCR). The nucleotide and deduced amino acid sequences for VP2 hypervariable region of selected five IBDV field isola...
Abdul A. Mirani1,2*, Chee H. Teo2, Adel A. Abul-Soad3, Ghulam S. Markhand1, Tahira Jatt1, Ameer A. Mirbahar1,4, Najamuddin Solangi1
...ve not been reported for tissue-cultured date palm (Phoenix dactylifera) plants derived from immature inflorescences. In this study, three to four years old field grown tissue cultured date palm plants of cvs. Kashuwari and Gulistan derived from in vitro subculture 1-10 (block I) and 11-25 (block II) in multiplication stage were screened for type and nature of phenotypic abnormalities. Six phenotypic abnormalities were detec...
Uzma Jabeen1, Irfan Khan2, Nadia Naeem2, Asmat Salim2,* and Waseem Ahmed1
...toxic effects on healthy tissues. Doxorubicin (DOX) is a commonly used chemotherapeutic drug. Oxidative stress plays an important role in DOX-induced toxicity. This study was aimed at analyzing the expression levels of genes that are induced in response to oxidative stress in heart and liver tissues following DOX administration to rats. In this study, DOX was administered to rats intraperitoneally (i. p.) at 3 mg/kg at alter...
Zeynep Soyer Sarıca1*, Meryem Eren2 and Meryem Senturk2
...tive damage in the brain tissue of Sprague Dawley rats. Malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) enzymes were estimated in the brain tissues of the Sprague Dawley rats. Compared to the control groups, a significant increase in the brain tissue MDA level of the group which received only 10 mg/kg K2
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
...haracterize FAdVs. Liver tissues were collected from poultry flocks showing clinical signs of HHS and investigated using PCR assay for FAdVs. The FAdVs were detected in 8 samples collected from different flocks. Positive PCR amplicons were sequenced to genetically identify the FAdVs. Phylogenetic analysis revealed two distinct clusters among FAdVs. One cluster containing 3 strains belonged to the FAdV- C species and serotyped as FAdV-4 and showed close proximi...
Nouf Alharbi*, Mai Elobeid and Promy Virk
...g Cd accumulation in the tissues, reversing the effects of Cd toxicity on blood profile, and on the CAT and SOD activity in the liver and decreased the MDA levels in both serum and liver. Thus, the key findings suggest a profound antioxidative potential of the low dose of querectin, which could be a prospective approach in the treatment of Cd intoxication.
...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1, Muhammad Faheem Malik3 and Muhammad Idrees4
...fluids, liver and spleen tissues were collected from overnight dead layer chickens (n = 8) from Pattoki region of Punjab province, Pakistan, during July to August 2016. The RT-PCR showed the amplifications of selected regions of helicase (186 bps, 2769-2954 nt) and capsid (280 bps, 5461-5741 nt) domain in the bile fluids of two birds. The histological data demonstrated pathological changes in liver and spleen tissues of laye...
Maliha Ayub1, Muhammad Javed1, Fariha Latif 1*, Faiza Ambreen2, Komal Sabir1
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
... be expressed in cumulus tissue, granular tissue, hypophysis, genital ridge and tissues of ovary and testis. Moreover, QRT-PCR results showed that BMP15 was expressed in the whole process of embryogenesis and folliculogenesis, early high level and then down regulated. It was significantly expressed higher level in COCs of middle diameter sized follicles than that of small and large ...
Saleh S. Alhewairini
...ect spray or soaked palm tissue (contact/ingestion)) was significant (p < 0.05) except the for difference between 1 h and 24 hin larvae with direct spray treatment, which was insignificant (p > 0.05). The study concludes that new information regarding the efficacy of these two insecticides which can be used to replace the conventional, over-aged and less effective insecticides that have been extensively used in controlling RPW.
... 
Aqsa Pervaiz1*, Rukhshanda Afridi1, Zahra Pervaiz1, Rabia Masood1 and Hira Pervaiz2
...eneration of hemopoietic tissue, destruction of hepatocytes, degeneration of bile duct, inflammatory infiltration, vacuolization, pyknosis, presence of MMCs and necrosis in hepatocytes. It can be concludedthat methylmercury has harmful effects on histology of liver of fish. Therefore, the present work suggests that the exposure to the methylmercury should be prevented to avoid injurious health hazard risks to fish.
...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
... lesions of all examined tissues. Strong positive staining was primarily found in the spleen, liver, and testicle. In conclusion, this study was the first to report the isolation of two B. canis strains from pet dogs in Sichuan province, southwestern China, and to further evaluate B. canis antigen location in tissues. Our study will contribute to the understanding of B. canis pathogenicity in naturally-i...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*
...pression may cause liver tissue damage. At present, few studies on CYP1A1 methylation and anti-tuberculosis drug-induced liver injury are available. Studying the relationship between CpG island methylation in the CYP1A1 promoter region and drug-induced liver injury is, therefore, of great importance. Anti-tuberculosis drugs induced hepatotoxicity in HL-7702 cells was investigated. Dehydrogenase activity was detected by a lactate dehydrogenase assay kit; supero...
Shou-Hong Zhang1*, Ying Zhang1, Kun-Ming Wen2, Wei Wu3 and Wen-Yan Li3
...stage. The total fibrous tissue in the liver of rats treated with HGF was lower than that in control group. The time-signal intensity curve (TIC) peak of liver parenchyma of rats with hepatic fibrosis was characterized by a slow wash-in –out. A correlation was found between the perfusion parameters and the stage of fibrosis. As the stage of fibrosis increased, the wash-in and -out rates decreased progressively. The use of Bio-MB+Bio-CNLP is an effective ...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1
...atic growth, metabolism, tissue repair, lactation and reproduction. It controls differentiation and maintenance of different function in various and specific tissues. The liver is the primary source of IGF-1 and considered as candidate genes for growth because they play a significant role in development and growth regulation. IGF-1 control protein metabolism and is extremely conserved region amongst species. IGF-1 have not b...
Nosheen1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1
...xidase (POD) activity in tissues viz. liver, gills, kidney, muscle, heart and brain of fish Catla catla exposed for 4-day. After each sampling (24-hr interval) fish were dissected and required organs were obtained. Results showed that the POD activity was augmented in all selected organs of Pb+2 exposed fish in relation to control. The POD activity in organs of fish followed the order: brain>liver>gills>kidney>heart>muscle. Regression analysis s...
Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2
... B. napus floral-dip and tissue culture based approaches were tested using different formulations of phytohormones for calli, shoots and roots induction in a variety of genotypes. While false positive lines were obtained in the result of floral-dip method, the tissue culture based method led to successful regeneration of shoots on Murashige and Skoog (MS) media containing BAP 3 mgL-1 and developed roots in ½ MS media ...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
...tial oxidative injury to tissues and lead to cartilage degradation in RA patients.
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Hafiz Muhammad Tahir*, Palwasha Jabeen, Chand Raza, Shaukat Ali
...application in culturing tissues including skin, bone, cartilage and nerves. Sericin has been hailed to have antitumor activity and has also been widely used in different cosmetics. The major hindrance in using spider silk for different purposes is due to its small obtainable amount. Efforts are being made to overcome this problem by modern biotechnological techniques including of making transgenic organisms.
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Fariha Latif1*, Muhammad Javed2, Hammad Ahmad Khan2 and Khalil-Ur-Rehman3
...accumulation in the fish tissues that increased significantly with increasing exposure of Pb. Pb accumulated variably in the fish tissues as liver > gills > brain > kidney > muscles. Oxidative stress in the Pb exposed fish was determined in terms of change in the activities of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). The Pb exposure caused a significant time and do...
Hazrat Ali1,2,*, Ezzat Khan1,* and Muhammad Jamal Nasir3
...as also studied in other tissues such as skin, gills, liver and kidneys of two fish species i.e., Clupisoma naziri and Mastacembelus armatus. Cr concentrations in fish muscles ranged from 30.5±41.9 to 70.8±12.1 mg kg−1 wet weight, Ni from 16.7±10.2 to 103.5±114.1 mg kg−1 wet weight, Cd from 1.1±0.18 to 2.5±0.21 mg kg−1 wet weight and Pb from 29.7&p...
Kadry A. El-Bakry1, Lamiaa E.M. Deef1*, Lotfy Z. Habbak1 and Samia S. El-Naeli2
...mical analysis and liver tissues were collected and fixed in 10% formalin for histopathological studies. The present study showed that, PAT causes elevated alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and significantly increases serum level of malondialdehyde (MDA) but decrease the activity of superoxide dismutase (SOD) enzyme. On the other hand, in patuilin injected rats and treated with ginger, the activity of ALT, AST and S...
Sameh S. Tawfik, Ahmed A. Elkady* and Ehab T. Mohamed
...ogical-findings of renal tissues in the irradiated-group, showed nephrotoxicity; the renal cortex showed massive necrotic changes, the convoluted tubules showed distinctive pattern of ischemic renal injury and the cuboidal epithelium cells of proximal and distal renal tubules showed nuclear changes with leukocytes infiltrations. More over some cases showed atrophied glomeruli, widened Bowman’s space and thickened basement membrane, while in TSE-treated a...
Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2, Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1, Aneela Zameer Durrani4 and Muhammad Usman4*
...jab, Pakistan. Different tissue samples were collected from 560 cattle showing respiratory signs at slaughter. Lung samples were used for detection of CBPP through PCR targeting 16S ribosomal RNA. Furthermore, lungs, liver, kidney and lymph node were collected for histopathological examination. Of the 560 samples, 49 (8.25%) were found positive for CBPP. Maximum pathological lesions (50-75% of surface area) were seen in 34.69 percent lung samples, chronic type...
Ramazan İlgün1*, Nilgün Kuru2, Ferhan Bölükbaş3 and Fatih Mehmet Gür4
...g a light microscope the tissues and papillae of the tongue were examined with a the scanning electron microscope, and photos of the general histologic structures were taken. The tongue was triangular shaped, and consisted of apex, corpus, and radix sections. The dorsal and ventral surfaces of the tongue were covered by a keratinised stratified squamous epithelium. Lamina propria and submucosal layers were distinguishable underneath the epitelium. SEM observat...
Gasem Mohammad Abu-Taweel
...tocytes, polymorphism of tissue, nuclei and vessel congestions when compared with control group. The development, behavioral, biochemical and histological disorders were observed due to exposure to mercury via placenta during pregnancy and / or during lactation through weaning period. Overall, mercury chloride administration had direct influence on the social behavioural and blood parameters in the mice offsprings.
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