Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
... effect and mechanism of spermidine have been reported, the role of spermidine-induced autophay in cancer cell survival has not been fully elucidated yet. In the present study, we aim to investigate the effect and toxic mechanism of spermidine on cell survival using Hela cells, a cervical cancer cell line as a model. Cell viability was assessed by MTT assay. LDH leakage was determined usin...

Gulzar Ullah* and Gohar Ayub

...tration (193.95 nmol/g), spermidine concentration (154.59 nmol/g), spermine concentration (36.51 nmol/g), yield (20.14 t ha-1), early flowering (26.99 days) and lowest electrolyte leakage (48.87%) was observed in plants treated with 20 hours heat pretreatment duration as compared to other heat pretreatment durations and control. Concerning tomato varieties, number of flower clusters plant-1 (16.99), fruit set percentage (68.25%), number of flowers cluster-1 (7...

Xian Zhang1, Erjiang Lin1, Shizhe Hong1 and Zhengjie Zhu2*

...nvestigate the effect of spermidine/spermine-N(1)-acetyltransferase (SSAT) on the proliferation and apoptosis of prostate cancer LNCaP cells under hypoxic conditions. Prostate cancer LNCaP cells were transfected with the eukaryotic expression plasmid pcDNA3.1 (D)/V5-His-SSAT, and cultured at 1% oxygen concentration. Western blotting was used to detect expression of SSAT, Akt, GSK-3β and β-catenin proteins; and flow cytometry was used to detect cell a...

Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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