Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa
...) on digestion with AsnI restriction enzyme further confirming the isolates. This is the first report of the isolation and molecular identification of Mmm from the ear canal of cattle in Nigeria. It is recommended that samples from the ear canal be considered when Mmm isolation is intended so as to improve the chance of recovery. ...
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...ted for RAPD-PCR and PCR-restriction enzyme analysis. Mycobacterium bovis (M. bovis) and M. tuberculosis H37Rv were used as reference strains. RAPD-PCR differentiated the strains and authenticated the high molecular diversity. PCR-restriction enzyme analysis (PRA) profiles of all isolates and reference strains were found to be the same. PRA is a valid alternative to phenotypic identification in a ...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
...lasmid was identified by restriction enzyme and sequencing analysis. The in vitro expression of the truncated protein was analyzed in E. coli with a molecular weight of 38kDa determined through SDS-PAGE and confirmed by Western blotting. The recombinant truncated protein was then purified from the culture media. The immunogenicity of the protein was studied in an animal experiment on mice, in which mice were injected subcutaneously. These f...
Memis Ozdemir
...detected the RsaI restriction enzyme digestion site in Prl gene exon 3 region reported by many literature and, these results also have been approved by blasted at the NCBI Genbank.
... 
Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3
Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis
...hat were restricted four restriction enzymes (HhaeI, MboI, BsuRI, HinfI). PCR ITS-RFLP banding pattern identified two Fusarium species i.e. Fusarium oxysporum and Fusarium solani. In conclusion, PCR-ITS-RFLP proved to be an efficient and easy technique for the identification of wilt complex pathogens....
Mohamed I. Azzam1, Safaa M. Ezzat1, K. A. El-Dougdoug2, Badawi A. Othman2
Tiara Widyaputri*, Aldryan Cristianto Pratama, Dian Vidiastuti, Yudit Oktanella
...analysis of NCOI cutting restriction enzymes shows that there are two bands at 66bp and 40bp. This shows that the normal allele frequency is one and the mutant allele is 0 with a heterozygous genotype frequency of 0% in the population. According to the result, the polymorphism that appears in the CD18 gene by PCR-RFLP method in all samples shows the substitution mutation type G base to C transversion and is not related to the BLAD mutation.
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Ly Thi Thu Lan1, Nguyen Thi Anh Thu1, Lam Thai Hung2, Nguyen Thi Hong Nhan3, Le Thanh Phuong4, Nguyen Trong Ngu3*
...ed with the help of AcyI restriction enzyme. A polymorphic site was found in the population, with the frequencies of 11, 12, and 22 genotypes being 0.18, 0.67, and 0.15, respectively. At the end of the experiment, better body weight (965-976 g/bird) and daily weight gain (10.5-10.6 g/bird/day) were found in chickens with 11 and 12 genotypes. Carcass parameters were superior in chickens carrying these genotypes (P<0.05) except for thigh and drumstick weight....
Tri Eko Susilorini1*, Ahmad Furqon2, Wike Andre Septian1, Desinta Wulandari3, Suyadi Suyadi3
Genevieve R. Tabon1,2, Noraine P. Medina2, Mary Rose D. Uy-De Guia3, Claro N. Mingala2,3*
...cted to RFLP using PvuII restriction enzyme. Results showed that all of the samples were found to be of AA genotype which is not the favorable genotype according to previous studies. The litter sizes of the PNP were also determined. Results showed that the genotype of ESR1 gene present in PNP is homozygote AA. The average litter size of the 30 PNP is 7.07 which is relatively higher than the average litter sizes of Nepal native pigs which ranges from 2 to 6. Mo...
Muhammad Ismail1, Jabbar Khan1*, Zahid Rauf2, Khalid Khan3 and Muhammad Rafi1
...sted with Xbal and Pvull restriction enzymes and were electrophoresed to visualize single nucleotide polymorphisms (SNPs); rs9340799 (G>A) and rs2234693 (C>T) in ESR1 gene. The amplified product of 524bp of rs9340799 sequence of ESR1 gene was treated with Xbal restriction enzyme while amplified product of 451bp of rs2234693 sequence was treated with PvuII restriction enzyme. Nine pat...
