Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2
...oled morbid material and fecal samples from 600 different locations across the region were analyzed for isolation. Isolation was carried out on embryonated chicken eggs. Identification of H protein was undertaken through hem agglutination Inhibition (HI) test while N typing was done using reverse transcriptase polymerase chain reaction (RT-PCR) procedure. Out of 600 examined locations twenty two (22) H9N2 subtypes isolates were recorded. ...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
... extracted from positive fecal samples for coccidian parasites and a T. gondii PCR was performed. Five sets of primers were designed using PrimerSelect tool for PCR to amplify SAG2 gene 5’ and 3’ regions. Sequences of 5 fragments of SAG2 were annotated and analyzed using DNASTAR Lasergene. After phylogenetic analysis with 3 clonal types and atypical strains, and on the basis of restriction map of HhaI and Sau3AI, our 3 isolates of T. gond...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*

 

.../i>were isolated from 83 fecal samples. The organisms were inoculated on MacConkey agar and EMB agar and identified via biochemical tests. Polymerase chain reaction was used to detect representative virulence factors or genes, including E.coli adherence factor (K88, CS31A, afaE-8), toxins (estA, estB, Stxl, Stx2, EAST1), pathogenicity island (eaeA, irp2, ETT2) and outer membrane protein (ompA). Moreover O-antigen serot...
Hafiz Muhammad Ishaq1, Muhammad Shahzad2, Xiaokang Wu3, Chaofeng Ma4 and Jiru Xu1,*

Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2* 

...d. In this study, canine fecal samples from diverse States of Brazil were screened by PCRs for CCoV and positive samples were subjected to partial sequencing for the membrane (M), spike (S), nucleocapsid (N) and non-structural protein 3b genes. Out of the samples collected, 40.17% were positive for CCoV; 57.45% of CCoV-infected animals showed enteritis and most of these (76%) were younger than 3 months and unvaccinated. Distance genealogy using CCoV sequences ...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1Nisar Ahmad3, Ayesha Hassan1, Muhammad Kashif Prince1, Muhammad Luqman Sohail4,*, Mian Saeed Sarwar2, Hazrat Ali2, Tahir Usman2, Asadullah Khan2, Siffat Ullah Khan3 and Shahid Zaman5
... Three hundred and sixty fecal samples (n=360) were collected through convenient sampling from apparently healthy lambs and were examined using the modified Ziehl Neelsen acid fast staining. Prevalence was calculated along with different risk factors associated with the disease (month, age, sex and season). Results showed highest prevalence (P < 0.05) of the Cryptosporidium in Kohat (33.33%) followed by Lakki Marwat (25%), while the lowest percent pr...
Jun Chen1, Shuai Shang2, Xiaoyang Wu1, Jiakuo Yan1, Huaming Zhong1, Huanxin Zhang2, Weilai Sha1, Wanchao Zhu1 and Honghai Zhang1,*
... rRNA gene obtained from fecal samples from three cape oryx animals, one female and two males. At the 3% level in our research, we found 3959, 4553, and 3930 operational taxonomical units (OTUs). Additionally, the three samples have 754 OTUs in common, which comprised 19.59%, 16.55%, and 19.81% of the reads in C1, C2 and C3. We identified 18 prokaryotic phyla in these animals, but most of the gut flora belonged to three phyla: Firmicutes (42.81-55.29%), Bacter...
Imran Taj1,*, Ferhat Abbas1, Zafar Ahmad1, Mohammad Kamran Taj1, Deedar Ahmad2, Zahoor Ahmed2, Ghulam Mohammad2, Ajaz-ul-Haq1, Farooq Shahzad1 and Sabiha Azam3
...uminants. A total of 204 fecal samples were collected from sheep and goats suffering from seasonal diarrhea. C. perfringens type D was identified through different biochemical tests and PCR. Antibiograms and lab animal trials were also carried out on local isolates of C. perfringens type D. Out of 204 analyzed samples, 89.18 % were found positive while 10.82 % were negative for C. perfringens type D. The division wise distribution showed t...

 Asad Sultan1, Rabia Ali1, Rifat Ullah Khan2,*, Sarzamin Khan1, Naila Chand1 and Ambrina Tariq3

...ate content in grain and fecal samples. Sorghum cultivars were different in nutrient profile with red higher in protein content (11.41%). It was observed that phytase inclusion in grain increased the availability of all nutrients except crude lipids. Total tract nitrogen retention was increased by 3% in red sorghum compared to white. Minerals absorption was increased but differently in different cultivars with higher degradation of phytate in both red and whit...

Muhammad Sohail1*, Asad Sultan2, Said Sajjad Ali Shah3, Muhammad Sajid1 and Adnan Khan4

...ps for the collection of fecal samples and further analyses. Nutrient profile, digestibility coefficients, and apparent metabolizable energy were investigated. Fat percentage and metabolizable energy was higher in maize (4.1% and 17.04 MJ/kg). The protein levels in wheat varieties were 12.15 and 11.89% which were more than that of both of corn (8.21 and 8.05% respectively) and sorghum varieties (10.41% and 9.89% respectively). The bioavailability of crude prot...
Fiza Asif1,*, Muhammad Siddique Awan1, Nasra Ashraf1, Nuzhat Shafi1, Abdul Rauf1, Khizra Bano1, Muhammad Razzaq2 and Naeem Iftikhar Dar2
...logical technique. Sixty fecal samples were collected from the study area during summer 2015 and winter 2015-16, i.e. thirty samples during each season. These samples were analyzed for the determination of food composition using microhistological technique. A total of 23 plant species were observed during summer and 15 plant species during winter season. During both the seasons Indian or Himalayan Chestnut Aesculus indica was found as the dominan...
R. Yasmeen1*, S. Shaheen1, B.N. Khan2, S.S. Bokhari1, U. Rafi1 and A.W. Qurashi1
...n feed were reflected in fecal samples; however, higher amount of Pb and Cd were noticed in fecal samples compared to feed, water and soil. This showed inhalation of these metals from the air. Amount of metals at different depths showed unique pattern low to high and again low that was irrespective of site location. Overall there was more exposure to air pollutants noticed for Lahore zoo compared to Bahawalpur zoo.
Wen Qin1,2, YanGan Huang1, Lei Wang1, Gonghua Lin1, Jundong Yang1,2, Pengfei Song1,2, Hongmei Gao1,2, Jingjie Zhang1,2 and Tongzuo Zhang1,3,*
...of the gut microbiota in fecal samples from wild goitered gazelle from Qiadam Basin, Qinghai-Tibet Plateau, which are the first such data to be reported. A total of 25 phyla were found, of which the dominant phyla are Firmicutes and Bacteroidetes in both summer and winter, representing more than 90% of the overall relative abundance. Diet is a key factor in shaping the gut microbiota. Because of large differences in host diet between summer and winter, there a...

M. Hammouchi1*, G. Sebbar1, N. Touil2, C. Loutfi1, Y.S. Malik3, M. El-Harrak1 and O. Fassi-Fehri4 

... detectable in swabs and fecal samples taken at the peak of infection. The transmission of the disease to animals (Timahdid breeds) brought into contact without manifestation of clinical signs, may indicate that local breeds of sheep might play an important role in the prevention of dissemination of PPR by virtue of its natural immunity. 

...

Md. Aminul Islam1*, A.N.M. Aminoor Rahman2, Mohammad Shah Alam3 and Md. Taimur Islam4

Gastrointestinal Nematodiasis in Quail in Bangladesh
...ngladesh. A total of 200 fecal samples were collected from quail farms from various regions of Bangladesh. The fecal samples analyzed by direct smear methods and nematodes identified by the presence of characteristic eggs in the feces. Five types of nematode eggs found on different farms. The overall prevalence of gastrointestinal nematodiasis were (17.5%), which could consider relatively higher. The highest rate of infectio...

Sidra Shehzadi, Sher Bahadar Khan*, Umar Sadique and Saqib Nawaz

Isolation and Molecular Identification of Clostridium perfringens Type D in Goats in District Peshawar
...cination. A total of 100 fecal samples  were collected aseptically from four different zones of district Peshawar during the period of February, 2019 to April, 2019. C. perfringens  Type D was confirmed through bacterial culturing, Gram staining, biochemical tests and polymerase chain reaction (PCR) . Results revealed that 25 feacal samples collected from suspected goats were positive for C. perfringens  Type D  and the overall prevalence o...

Said Sajjad Ali Shah1*, Muhammad Ilyas Khan1, Aziz Ullah1, Hayat Ullah2 and Faisal Ahmad2

Coprological Examination of Small and Large Ruminants in Central Zone of Khyber Pakhtunkhwa
...purpose, a total of 1700 fecal samples were received from central districts and processed for coprological examination. Out of 1700 samples, 947 samples (55.7%) were positive for intestinal parasites. Specie wise prevalence of GI parasites was recorded as 51.5% in cows, 58.3% in buffalo, 73.9 % in sheep and 68.5% in goats. Trichostrongylus (32.8%) was recorded as highly prevalent in cattle followed by amoeba (10.2%) and Fasciola (6.1%), while in buffaloes fasc...
Arnab Tanveer, Shabana Naz*, Azhar Rafique and Asma Ashraf
...alnagar. Freshly dropped fecal sample were collected once before treatment with anthelminthic drug and twice after treatment and brought to laboratory for qualitative and quantitative analysis. Direct smear method and fecal floatation technique was used for isolation and identification of endo-parasites. Modified McMaster technique was used to calculate Eggs per gram. The data was subjected to ANOVA and Tukey-HSD (post hoc test). Six species of endo-parasites ...

Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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