Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*

...tion. The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years and more than 2 years for both ELISA and SNT. The peak immune response measured by SNT was 2.8log10 whereas ELISA detected serum antibody titer up to 2.4 log10. Findings showed that highest titers were seen in animals of age > 2 years i.e. 1.70 log10 (for ...

Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren

...tial to know if estimated antibody levels vary over a period of weeks or months. Therefore, the aim of this study was to evaluate the short term stability and the long term consistency of the antibody levels to Aleutian disease virus (ADV). When evaluated in the short term, i.e. between consecutive days and weeks, the estimated antibody levels were comparable and did not exceed the predefi...

Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*

... genetics, anti idiotypic antibody based vaccines and helper cell dependent diagnostics represent the present vaccine/diagnostic development strategies for the effective control and eradication of PPR. This review comprehend the current scenario of recombinant technology based vaccines and diagnostics, virus like particles (VLPs), reverse genetics approach etc., in the development of diagnostics and vaccines against PPR.

...

Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2

...dy aimed at measuring the antibody titres against H9N2 influenza viruses and its molecular detections in asymptomatic commercial layers from Faisalabad district of Punjab, Pakistan. Overall 120 blood samples, 24 tissue samples from each organ (trachea, lung and intestine) were collected from the 12 commercial layer flocks selected randomly at the age of 35-50 weeks without any direct clinical manifestation of avian influenza. Serum collected from 120 birds was...

Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar

...PPRV antigen to determine antibody titres level. The overall prevalence of antibodies to PPRV in goats and sheep were 66.22% (521 samples) and 65.76% (444 samples), respectively. Sero-prevalence of anti-PPRV antibodies was found higher in adults (69.14% with GMT 17.91) than in young (62.47% with GMT 12.85). Furthermore, seasonal seropositivity seasons was recorded and sero-prevalence during summer was found to be higher (72.84% with GMT 20.18) than other seaso...

Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5

...exhibited similar pattern antibody profiles, however, an increase in the mean titer of antibodies was recorded from 17 and onwards. Furthermore, on day 39 of age, the birds of all groups including control were challenged with field strain of IBD virus collected from bursa of clinically infected chicks, No morbidity and mortality were observed. During present study, some milder infection with field strain of IBDV almost a week ago recorded were also found in th...
Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
...rabies virus neutralizing antibody titer (mRVNA). All puppies were whelped by dams regularly vaccinated with high quality inactivated rabies vaccines. Rapid Fluorescence Focus Inhibition Technique (RFFIT) revealed mRVNA titer (>0.5 IU/mL) in 29 puppies while 48 puppies showed mRVNA titer less than minimum protective level (0.2-0.4 IU/mL). RFFIT did not reveal mRVNA in 23 puppies that were declared lacking maternal rabies immunity. One basis of findings of t...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...ekly post vaccination for antibody detection by HI test. All immunized chickens shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post-challenge (p.c.) for virus titration in eggs. Data indicated that all chickens vaccinated wi...

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
...ing, electron microscopy, antibody-based detection, including ELISA, nucleic acid hybridizations like dot blot, squash blot and Southern blot techniques, polymerase chain reaction and other DNA polymerase-mediated assays, and microarray detection. Of these, microarray detection provides the greatest capability for parallel yet specific testing and can be used to detect individual or combinations of viruses with sensitivity comparable to ELISA. Methods based on...

Shams Ur Rehman, Muhammad Arif and Abdul Mateen 

 

Soil-borne viruses in major potato growing areas of Pakistan
...ctivity assays and double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Both the viruses were prevalent in Hazara and Malakand divisions. The average symptoms based incidence in Hazara divi-sion for PMTV was 8.83% and TRV was 6.80% while in Malakand division the average symptoms based inci-dence for PMTV was 8.46% and TRV was 9.95%. DAS-ELISA based average incidence in Hazara of PMTV and TRV was 17.34% and 11.99% respectively. The average DA...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...e drops. Group B specific antibody negative (SAN) chicks were challenged with same dose at 2nd week of age. While the group C and D was unchallenged control containing commercial and SAN chicks. The Blood samples were collected from group A, B, C and D on 3rd, 5th and 7th day of post infection for biochemical analysis. The Albumin and total protein values were significantly low (P<0.05) in infected groups A and B ...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...s developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmid DNA vaccine. The Egy-H5 plasmid DNA vaccine induced complete protection (100%) and there was no virus shedding in all chickens immunized with the Egy-H5 plasmid DNA vaccine. Taken together, the...
Naila Chand1,*, Shabana Naz2, Hamza Maris1, Rifat Ullhah Khan1, Sarzamin Khan1 and Muhammad Subhan Qureshi1
...hil/lymphocytes ratio and antibody titer was significantly (P<0.05) lower in the treated groups. Based on these results, it is concluded that supplementation of diets with betaine improved broiler feed intake, weight gain, FCR and increased immunity were under heat stress condition.
...

Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1 

 

...ccine A showed mean ELISA antibody S/P ratios from 0.099 to 0.995 between weeks 2 and 6 post-vaccination against IBV antigen, while for vaccine B group mean S/P ratio dropped to 0.550 at week 6. Virus neutralization test showed that birds vaccinated with vaccine A showed neutralizing antibodies against IBV Massachusetts, 4/91, D274 and Qx types, while titers for vaccine B were not detected, suggesting a role of the poor emulsion quality on the performance of v...
Kashif Prince1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Atif Prince3, Naimat Ullah Khan1 and Mumtaz Ali Khan1
...sults showed that highest antibody titer was developed in cattle, colostrum and their calves in group G in which treatment was started 75 days prepartum and VESS was given. It was concluded that vitamin E and selenium supplementation improves both active and passive immune system if given before 75 days of parturition.
...
Zia-ur-Rehaman1, Naila Chand1, Sarzamin Khan1 and Rifat Ullah Khan2,*
... day 21 to 42. Mean serum antibody titer against Newcastle disease (ND) and serum paraoxonase (PON1) were significantly higher (P< 0.05) in TN zone, while serum malondialdehyde (MDA) was significantly (P< 0.05) higher in HAT zone. No significant differences were recorded in serum antibody titer against ND, PON1 and MDA among different broiler strains in TN zone, while in HAT zone, Ross and Arber Acer showed significant...

 Javid Iqbal, Asghar Ali Mian, Tanveer Ahmad*, Shamsul Hassan** and Sohail Hassan Khan* 

COMPARATIVE PERFORMANCE OF DIFFERENT ECONOMIC TRAITS OF FOUR IMPORTED BROILER STRAINS UNDER LOCAL CONDITIONS OF PAKISTAN
...n ratio (FCR), mortality, antibody titres, dressing percentage and economic evaluation as cost of broiler per kilogram live weight produced. The average body weights gained by the birds were 2264g, 2277g, 2256g and 1942g for A, B, C and D strains, respectively at 49 days of age. The birds in treatments A, B and C gained significantly (P <0.05) more weight than the birds in treatment D. On the contrary, the birds in treatment D (4371g) consumed significantly...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...Most animals had high IgG antibody titers to CPV prior to vaccination and vaccination did not result in increased titers. A decline in CPV antibody titer with increasing age was observed regardless of immunization status. Our results suggest that vaccination of African wild dogs with a live attenuated domestic dog vaccine may provide adequate protection against CDV, and could therefore be an important tool for the conservati...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...utination inhibition (HI) antibody titer (MT log2) against AIV H9 remained 7±3. Real-time PCR results showed 8.3% (42/507) of the samples were positive for viral matrix gene. Sub-typing was performed by specific anti-sera. PCR amplification was done by using N2 specific primers that confirmed 100% of the matrix (M) gene positive isolates as subtype H9N2. This is the first report from Pakistan that confirms prevalence of AIV H9N2 among differe...

Ghufran ul Haq1*, Muhammad Arif2, Asad Ali2 and Mian Inayatullah3 

...ion of its vector. Triple antibody sandwich-enzyme linked immunosorbent assay (TAS-ELISA) was successful in detecting TYLCV in naturally and whiteflies inoculated tomato plants. Transmission properties of the virus were determined by inoculation of tomato plants of susceptible cultivar, Roma-VF, by whiteflies, determining the minimum time required for acquisition and inoculation of the virus, latency period and the persistence capacity. The highest incidence o...
Hazrat Nabi1, Irshad Hussain2, Muhammad Adil3, Amar Nasir3,*, Arbab Sikandar3, Saeed Khan1 and Nasrullah Khan3

 

...t of mycotoxin binders on antibody titer against New Castle disease virus, feed consumption, weight gain, relative weights of lymphoid organs and feed conversion ratio (FCR) of broilers were investigated. Chicks fed aflatoxins (group B) alone manifested significantly reduced feed consumption, body weight gain and feed conversion ratio. However, no variation in weight gain and FCR was found between the controls (A, B and C) and mycotoxin binders fed groups (exp...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
... equal groups (n=18). The antibody titers were evaluated with indirect haemagglutination test. The results indicated significantly higher (P< 0.05) immune titer in animal group vaccinated with toxoid vaccine prepared from pathogenic isolates and provide best protection against challenge infection.
...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...ficantly stronger anti-SS antibody level than that in control (p < 0.05), and mice in T1 group induced significantly higher serum GH and PRL concentration in comparison with T2 and control groups (p < 0.05). Expectantly, mice in T1 group also showed significantly higher average weight gain and offspring weaning weight than that in T2 and control groups, respectively (p < 0.05). Overall, these results suggested that novel somatosta...

Ocheme Julius Okojokwu1*, Maryam Bisola Adebayo2, Bashiru Shafa Abubakar3, Ibrahim Abubakar Yusuf2 and Joseph Aje Anejo-Okopi1  

... (52.5%) of women had IgG antibody to Parvovirus B19 while 31 (9.5%) were positive for IgM antibody. Demographics including age group, educational status, occupation, type of marriage, number of children, history of blood transfusion and trimester of pregnancy were statistically associated with prevalence of IgG antibody (p ≤ 0.05). There was a significant association (χ2 = 12.571; ...
Tünay Kontaş Aşkar1,*, Şinasi Aşkar1, Olga Büyükleblebici2 and Murat Güzel3
...y the immune fluorescence antibody test with antibody titter ≥1:128 and lymph node smear examination. In blood samples of dogs with visceral leishmaniasis, levels of malondialdehyde, total antioxidant capacity, glutathione, nitric oxide, myeloperoxidase, adenosine deaminase and cytokines (TNF-alpha, IL-1β) were determined and compared with the healthy controls. Significantly high levels of plasma malondialdehyde, nit...

 Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1

Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...y, growth performance and antibody response in H9N2 challenged
broilers. A total of 80 chicks were divided in to 4 groups A, B, C & D. Group A and
B were challenged with AI virus (H9N2) on 21 day of age, group B and C were
treated with 3% FO while group A and D were treated with cooking oil. For isolation
of virus, 21 samples were collected, out of which 8 (40%) were positive for H9. The
virus titer was ranged from 1:64 t...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...nificantly higher anti-SS antibody, serum growth hormone and IGF-1 concentrations and average body weight than those in the control group (P < 0.05). Immunisation with ptCS/2SS-asd promoted the growth of rams similar to the effect of the simple co-injection of CpG and SS DNA vaccine. Thus, the fusion type of CpG adjuvant is an effective, simple, and low-cost method in enhancing the effect of SS DNA vaccine and the growth of ram lambs.
...
Ali Raza Jahejo1,2, Nasir Rajput2, Wen-xia Tian1,*, Muhammad Naeem2, Dildar Hussain Kalhoro2, Asmatullah Kaka2, Sheng Niu1 and Fa-jie Jia1
...n, and new castle disease antibody titer were significantly higher in basil supplementary group. The weight gain and feed conversion ratio significantly improved in basil treated group, while ascorbic acid and basil significantly decreased the water intake. Body temperature and mortality percentage were significantly decreased in ascorbic acid and basil supplementary group as compared to control. Dressing percentage significantly increased in both supplementar...

 Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*

...nd the immunofluorescence antibody invoved Sca-1, CD34, CD144, Desmin and CD45 which are the makers of MDSCs. Finally, MDSCs were induced into Adipocytes, osteoblasts, chondrocytes and neuron-like cells by the optimized inducing medium. After the two-step digestion and differential attachment technique, high purity MDSCs were successfully cultured. MDSCs were identified by round or short spindle cell, and gathered into clusters; The average doubling time and c...
Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*
...immune response, however, antibody response in T1 group were significantly stronger than that in T2 and C groups. Serum GH levels in T1 group was significantly higher than those in T2 and C groups, and serum PRL levels in T1 group was significantly higher than that in control group. Body weight of offsping rats in T1 group were significantly higher than that in control group at weeks 2 and 4 after birth, and body weight of offsping in T2 group were significant...
Rana Waqas Arshad1, Asim Aslam1, Muhammad Saeed Imran1, Kamran Ashraf2 and Raheela Akhtar1,*
...oups. ELISA revealed high antibody titre in live vector vaccine group and a partial protection was observed in birds vaccinated with immune complex or intermediate plus vaccines. Consistently, histopathological lesions of IBD were less evident in live vector vaccine group in comparison to other groups. In addition live vector vaccine improved the feed conversion ratio (FCR) by keeping the bird healthy and by decreasing the immunosuppression. These results indi...

Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5

...sAg) and Anti hepatitis C antibody (Anti-HCV antibody) after initial screening with no coinfection of both diseases. Out of 25 total infected individuals, 05(1.6%) were found HBsAg positive and 20(6.6%) were found anti-HCV positive. All the ICT positive individuals were further confirmed by quantitative Enzyme Linked Immunosorbent Assay (ELISA) and 23(7.6%) individuals were confirmed for both hepatitis B and C including 05(1...
Irfan Shahzad Sheikh, Masroor Ahmad Bajwa, Nadeem Rashid, Muhammad Zahid Mustafa, Mohammad Masood Tariq*, Majed Rafeeq, Abdul Samad, Tauseef M Asmat and Asad Ullah
...nted group had highest HI antibody titers (7.22±0.25, 7.36±0.18 and 6.55±0.38). In experiments 1 and 2, supplementation with immune modulators had significant (P<0.05) effects on total SRBC, IgG and IgM titers. In conclusion, vitamin E showed better immuno-modulatory effect followed by vitamin C, nucleotides and DNA, respectively. Supplementation of immuno-modulators at early age (1 to 21 days) showed more promising effect on immune per...
Gong Ga1,2 and SuoLang Sizhu2,*
...tive to anti-HEV IgM antibody and Seventeen serum sample (9.8%, 17/173) were positive for anti-HEV IgG antibody. The HEV RNA positive rate was of 4.6% (8/173) in feces and serum. Moreover, the prevalence of HEV RNA was 9.5% (2/21) in swine liver, 4.8% (1/21) in swine spleen, 9.5% (2/21) in swine kidney, 4.8% (1/21) in swine intestine and 14.3% (3/21) in swine pork. HEV antigens were abundantly observed in the ...
Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*
...A vaccines induced strong antibody response, significantly suppressed testosterone secretion and reduced scrotal circumference and testis weight. It also significantly reduced the concentrations of 4-methyloctanoic acid and 4-methylnonanoic acid in adipose tissue. These findings indicated that immunisation of oral and plasmid-injected KISS1 DNA vaccines can effectively suppress testosterone secretion, testis growth and mutton odour, and the novel oral <...
Samia Afzal*,Sadia Zahid, Iram Amin, Muhammad Shahid and Muhammad Idrees
...sitive, respectively. All antibody positive samples and a subset of negative samples (195) were further subjected to PCR for confirmatory purposes. Two different sets of primers were used for the identification of the viral genome. Thirty samples were found PCR positive and a subset of ten samples was direct sequenced. Sequence analysis showed 94% similarity to Stenotrophomonas maltophilia which is considered to be the second most prevalent bacterial sp...

Cornelius Arome Omatola*, Samson Daniel Iyeh, Shedrach James Abuh, Charles Kehinde Mofolorunsho, Martin-Luther Oseni Okolo and Phoebe Queen Akoh 

...treponemal and treponemal antibody test. Socio-demographic and obstetric parameters were obtained using structured questionnaires. Sixty-four (25.6%) out of 250 women had at least one STI. The overall prevalence of syphilis, HIV, HBV, HCV and HSV-2 were 28(11.2%), 21(8.4%), 10(4.0%), 5(2.0%) and 0% respectively. Women who were married, unemployed, aged 15-35 years and of low-income earners had a higher prevalence of STIs. Factors such as a history of STIs in w...
Shikun Ge1, 2, Yanli Yang3, Brindha Chelliappan4, 5, Antonysamy Michael5, Fagang Zhong1 and Xiaoying Zhang1, 2, 3, 4*
...on. Based on Based on IgY antibody titer, IgY thermostability, IgY acid and alkali resistance, resistance to trypsin and pepsin, four different IgY purification methods (PEG extraction, water dilution method, the effect of liquid egg yolk and egg yolk powder preparations) on the activity of IgY antibodies was investigated. The results showed that IgY was stable below 70 oC, and the activity was significantly lost when it exceeded 70 °C; IgY was ...

Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*

Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...as the direct fluorescent antibody (DFA) assay. This method is a combination of immunology and bio-imaging. This DFA assay is rapid and sensitive, but specificity is influenced by antibody production methods. Therefore, the aim of this review was to describe various antibody production methods, which might be used in developing DFA assay to diagnose H9N2 infection in humans or chickens, an...
Muhammad Tahir Khan1, Shahid Mehmood2*, Athar Mahmud2Khalid Javed3 and Jibran Hussain2

 

... biochemistry, and immune antibody response. A total of 1200 newly hatched quail chicks (Coturnix coturnix japonica) were randomly allocated to five treatment groups. Each treatment group contained 40 birds and experiments were replicated six times using a completely randomized design (CRD). The experimental diets consisted of increasing levels of compost (0, 2.5, 5, 7.5, and 10%), but were otherwise iso-caloric and iso-nitrogenous. Data were analyzed b...
Neelam Pery1*, Fatima Ijaz1, Nayab Batool Rizvi1, Munawar Ali Munawar1 and Muhammad Imtiaz Shafiq2*
...m cytokine expression and antibody production to the bone resorption and cartilage destruction. We propose a dual trap to control this pathogenesis in the form of dual JAK3/BTK inhibitor. Using the computer-aided drug designing techniques, we developed dual JAK3 and BTK inhibitors based on quinoxaline derivatives which show appreciable dual inhibition in enzyme assays. To our knowledge, this is the first-ever report on dual JAK3/BTK inhibitors.
...

Masoud Hassani1* and Omid Madadgar2,3

Serological Evidence of Bluetongue in Iran: A Meta-Analysis Study
...etongue seroprevalence by antibody-captured ELISA test in livestock in Iran by searching terms including bluetongue, sheep, ovine, goat, caprine, cow, cattle, bovine, buffalo, camel, Iran and prevalence alone or in combination in both English and Farsi language. After reviewing 82 published articles, a total of 48 studies from 29 articles were eligible to be included in this meta-analysis study. The total seroprevalence of bluetongue in apparently healthy shee...
Muhammad Saeed Shah1, Fazal Nasir2, Sana Ul Haq1 and Muhammad Tahir Khan2*
...ion and suppression of an antibody in response to an antigen. Clonal selection theory represents how immune cells mutate and proliferate into memory cells. The methodology presented in this paper is autonomous and de-centralized. In the proposed system, the robots work effectively to complete a task either alone or co-operatively. Specifically, the coordination and communication among various robots are established based on the concept of immune system, idioty...
Rabia Riaz1, Khushi Muhammad1*, Masood Rabbani1, Muhammad Amjad Iqbal2, Aamir Riaz Khan2, Sadia Sarfaraz1, Mudassar Naseer3 and Khalid Majeed1
...ination for anti-MG-ELISA antibody titer. Cumulative mean anti-MG-ELISA antibody titer of birds to G-MG, O-MG, G-MG+NDV, O-MG+NDV and control was 80.30, 137.49, 91.59, 169.76 and 0.15 units respectively on 35 days post-vaccination. It is concluded that NDV has insignificant immunomodulatory effect on antibody response of birds to the MG vaccine.
...
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...ced by SCMP. The level of antibody titre against Newcastle Disease Vaccine was significantly higher (p≤0.05) than all treated groups. Higher antibody titre was recorded for group C (6g/Kg) (HI≥1:64) followed by group B (4g/Kg) (HI≥1:32) and Group A (2g/Kg) (HI≥1:8) while antibody titre was lower for control group D. Replacement of soybean meal with SCMP did not affect the level...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Irfan Shahzad Sheikh1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Khalid Mehmood1, Rana Muhammad Bilal2 and Tauseef Asmat1
... control (P<0.05). The antibody titer against Newcastle disease virus and sheep RBC showed no significant difference among treatment groups (P>0.05). Supplementation of herbal extracts as feed additives at 200 mg/Kg produced better growth performance of broiler chickens with no adverse effects.
...
Samina Tahir Kiani1, Abdul Rauf1, Syed Ayaz Kazmi1,*, Nuzhat Shafi1, Madiha Khalid2, Naeem Latif1, Samina Gul2, Saghir Yousaf3 and Muhammad Arshad4
...ere screened for anti-HCV antibody, HBsAg and anti-MTB antibodies using ICT kits. Sputum microscopy for MTB was done in MTB antibody positive samples. The samples positive in initial screening were confirmed by enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Nine samples were found positive in both ICT and ELISA test for HCV while PCR showed six positive cases for HCV. HBV were found positive i...

Walaa ,S. Shabanaa;Bakr, A. Abdallaa; Eman, M. ELShalamia; I.M. Redab; M. A. Shalabyb

...e.The
mean of antibody titers continued with the protective level till the 32 to 36th week post vaccination,
In single vaccination either FMD or RFV alone while till to 40th WPV in combined FMD/RVF
vaccine.In the final we can conclude that the use of Montanide ISA 50 as an oil adjuvant in prepared
vaccines improve the immune response against FMD and RVF, giving high titer of antibodies
against both di...

Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.

...tion of Hepatitis C Virus antibody and quantitative determination of RNA-HCV by RT-PCR were done. MnSOD activity was evaluated using a superoxide dis-mutase assay kit. Genotyping was performed by polymerase chain reaction and re-striction fragment length polymorphism (PCR-RFLP) method. The homozygous Ala/Ala (CC) was significantly more frequent in HCV-related HCC patients com-pared to HCV-infected patients and control group (67.5%, 12%, and 15% respective-ly, ...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...al tracts. Despite the H5 antibody responses in vaccinated chickens with program I (rFP-AI-H5" vaccine then Re-5) being significantly lower prior to challenge, it provided good protection (73.3%) against the lethal A/chicken/Egypt/SHAH-1403/2011 (H5N1) AIV challenge, with no evidence of virus shedding. Keeping in mind that each bird was potentially challenged with 105 EID50, which may not be available to all birds under field condition. The foremost vacci...

Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5

...orescence test. The serum antibody titers were determined by serum neutralization test (SNT) and Enzyme linked immunosorbant assay (ELISA) in calves and also their dams. The results confirmed infection of calves with lumpy skin disease virus (LSDV) and showed that calves older than 3 months old age and those out of first calf heifers are more susceptible for infection. Some calves even with insufficient amount of maternal antibodies could be protected from inf...

Walaa Abd El-Fatah S. Metwally1, Ismail M. Reda2, Ahmed A. El-Sanousi2, Mohamed Abd El-Khalek Ali2

...HA unit/dose were high in antibody titer than vaccines containing 256 and 100 HA unit/dose. The prepared vaccines with Montanide ISA 70VG containing 256 HA unit/dose gave high antibody titer than vaccines containing 100 HA unit/dose. The ratio of 30% Chicken strain of AI to 70 % Duck strain of AI gave the best result of antibody titer followed by the ratio 40% to 60% followed by 50% to 50%...

Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2

... and indirect fluorescent antibody technique (FAT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal diarrhea. Six out of forty tested samples were found positive for rotavirus (15%) infection using ELISA test. Successful isolation of the rotavirus on MDBK cell line from two samples from the positive ELISA samples. The beginning of characteristic cytopathic effects (CPE) were observ...

Hanaa A. Ahmed1 and Eman M. AboHatab2

...direct immuno fluorescent antibody technique (IFA) which was considered a rapid and accurate method for LSD antigen detection. nodules were subjected directly to PCR and Real time PCR for rapid and specific detection of LSDV, sixteen out of the twenty one nodular samples were confirmed positive by molecular methods. Eleven out of twenty one nodular samples were positive by (IFAT) in CAM, which prove that PCR and Real Time PCR are much sensitive and rapid diagn...

Yanni, M. I1; Hanaa A. Ahmed2; Lamyaa, A. Ahmed1; Hanan, A. Fahmy3 and Ibrahim, E.M.4 Aggour, M. G.3

...nt for direct fluorescent antibody technique and isolation on tissue culture using BHK cells. Direct confirmation was performed by Real time RT- PCR and gene sequencing was performed directly from brain samples. Phylogenetic analysis including sequences from all previous Egyptian isolates and neighboring countries isolates was conducted. The % of nucleotide identities of the Egyptian recent isolate (Egy/cairo /2014) with three previous Egyptian isolates were 9...

Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***

...superior as shown in mean antibody titers against Avibacterium paragallinarum serovar A, B and C and infectious bronchitis. In addition, Montanide ISA-70 is lesser in viscosity that makes it easily injected, so, it is better to use for control of infectious coryza and infectious bronchitis diseases in chickens.

...

1Mona A. El-Manzalawy, 2Abeer A. Boseila , 3Hanan M. El Zahed

...m gel only show the least antibody titer. The group of sheep, which was vaccinated with RVF, inactivated PMO without gel vaccine show intermediate level of antibodies. Moreover, the values of ED50 of the developed vaccines as well as the Alum vaccine alone were not exceed 0.02 which lies within the permissible level.

...

Serag eldeen Sultana, M.W Abdel azeemb, Nahla Mohamed Eldamranyc

Amira A. Mazyad1; A. A. Kheder1; Ahmed K. El-Attar1; W. Amer.2; Mahasen, H. Ismail.2; Amal, A.F.1

Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3

... SAT-2 with high titer of antibody against serotype (A) was 87 ,
serotype (O) was 72 and serotype (SAT-2) was 53 . Matching between SNT and ELISA was
applied for negative sera samples.
...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
...ers of virus-neutralizing antibody among the spectrum of EHV-1 proteins .A wave of Abortions has been recorded in El Zahraa stud for Arabian Horses ,collected samples were either aborted fetal tissues either Lung and livers as well as Aborted placenta and nasal swaps from mares suffering from repeated Abortions, The current study used the gene synthesis technology introduced by Invitrogen to synthesis the glycoprotein D of the Kentucky Strain as reference stra...

Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1

...ers of virus-neutralizing antibody among the spectrum of EHV-1 proteins based on sequence analysis and multiple alignment revealed single nucleotide substitution at the base pair number 121 from the start codon which give lead to single Amino Acid Substitution from CAG (Glutamine(Gln/Q)) which is considered as a polar Amino Acid to AAG (Lysine(Lys/K)) which is a basic Amino Acid which is very characteristic for the local isolate than the rest of isolate and st...

Megahed, A.A.1; Kh. A.El-Dougdoug2 and B.A. Othman2

...d serologically by double antibody sandwaich Enzyme linked immunosorbent assay (DAS-ELISA). The survey of sugar beet viruses achieved in 15 field locations belonging to 5 different Governorates, which performed by examination 915 plant samples from 149 fields in two seasons (2009/2010 and 2010/2011) symptomatology. During survey, 464 of naturally infected sugar beet plants showing distincted for one or more viruses were collected from 73 fields in season 2009/...

Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar

... high levels of CAV ELISA antibody titers in all the vaccinated chicken subgroups at 4th WPV with either DW or S/C CAV vaccine indicating that the two CAV vaccines were immunogenic; Also, by measuring H5N1 HI titers within the 6 weeks post vaccination with AI vaccine in the sera of different chicken subgroups using HI test. It is clearly observed that AI HI antibody mean titers in S/C and in DW CAV vaccinated subgroups at th...

Serageldeen Sultan1, Nabila Osman2

...the presence of avian HEV antibody using enzyme linked immuno-sorbent assay (ELISA). The results revealed that the occurrence of avian HEV was 15.7% (80/510) and 66.7% (16/24) of the examined flocks. HEV antibody prevalence increased as age dependent and most commonly occurred among broiler breeder flocks (10/24). In conclusion, the occurrence of avian HEV was confirmed for the first time among chicken flocks in Egypt.

...

Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed

... and indirect fluorescent antibody technique (IFAT) using specific hyper immune serum against LSDV. Further identifications were carried out by polymerase chain reaction (PCR) and clinco-pathological investigation. Results: The results showed that 11/30 biopsies were positive by AGPT, 19/30 by IFAT and 30/30 by PCR. While results of sero-diagnosis showed that 45/100 from apparently healthy non vaccinated cattle and 68/100 from vaccinated cattle were positive b...

Lamya A. F. Ateya1, Said A. Ahmed 2, Mansour H. Ayman3, Khamees K. Ashraf 4, Heba A. Abdel-Hady 5

...d by indirect fluorescent antibody technique (IFAT) using specific hyper immune serum against Lumpy Skin Disease Virus. Further identifications were carried out by polymerase chain reaction (PCR). Results: 15 samples were +ve for LSDV by conventional PCR.The results showed that 11/23 biopsies were positive by IFAT. Molecular identification of LSD virus by using RT-PCR, revealed positive for amplification of bands at the predicted molecular size (1926bp). Neutr...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...rgent to reach protective antibody titers and reduce virus shedding.
Methods: Groups of one-day old commercial broiler chicks were divided in to 8 groups 1, 2 and 3 were
vaccinated with a prepared vaccine contain 500HAU of H5N1 reassortant antigen; while group 4, 5 and 6
were vaccinated with an imported reassortant vaccine with 500HAU antigen content of H5N1 at 1, 5 and 10
days of age; respectively. A group 7 was...

Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2

... There was detectable TRT antibody titers by SNT in the first week post vaccination as 4 log2
in group-1 and 2 log2 in group-3. Both groups showed peak SNT titer (256 log2) of TRT virus by the
third-week post administration of the second dose and remain stable up to 24 weeks post vaccination.
Follow up avian influenza antibody titers using HI test, peak titer (64 log2) was detected in grou...
Allam A. Megahed, Badawi A. Othman, Samar S. El Masry, Abeer A. Faiesal and Mohamed A. Nasr Eldin

Abdelaziz A. Yassin, Ayman H. El Deeb, Abeer E. Mansour, Hussein A. Hussein

...-2, IL-4, IFN-γ and antibody response of calves vaccinated with conventional inactivated trivalent FMD vaccine. Expression of Th1 and Th2 was determined using quantitative ELISA. Results reveled peaks of IL-4 at 7th day, IFN-γ at 14th day and IL-2 at 21st day post vaccination. Lymphocytic proliferation assays demonstrated the continuous response of lymphocytes started at the 3rd day till reaches peak at 28th day post vaccination then start to decli...
Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
...
using double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Host range study
was carried out using mechanical inoculation into fourteen different diagnostic host plant species and
cultivars belonging to 6 families. Tomato seeds were treated with four different concentration of
NaN3and EMS (1.0, 2.0, 3.0 and 4.0 mM) and (0.3%, 0.4%, 0.5%and 0.6%) respectively, then planted
into a tis...

Wesley D. Nafarnda, Japhet H. Baraya, Olatunde H. Olabode, Malang K. Simon

...h significant (P<0.05) antibody titer increase from 1:16 - 1:1024 and mean
GMT 2.87 - 5.09 amongst 61.5 - 87.0% tested chickens. Questionnaire further showed V4
thermostable ND vaccination had significant effect on morbidity, mortality, hatchability, culling and
ultimately the flock productivity.
Conclusion: Corn-bran feed based vaccination demonstrated effective delivery and suitability of V4
ther...
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1
... higher RVFV neutralizing antibody titers.
Conclusion: The vaccine developed based on small particle vaccine formulation is an economic
solution that can be added inline to existing production platforms to enhance immunogenicity.
...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
...n this evaluation are the antibody immune response using hemagglutination
inhibition (HI) assay, protection post challenge, virus shedding and transmission in contact
non vaccinated chickens using qRT-PCR test.The genotype VII (GVII) vaccine group
induced higher HI titer by using GVII monospecific antigen and decreased virus shedding
than other group. The vaccinated group with genotype VII significantly reduced t...

Journal of Virological Sciences

July

Vol. 8, Pages 1-27

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