Protective Effect of Astaxanthin on Renal System in Rabbits Treated with Levofloxacin
Protective Effect of Astaxanthin on Renal System in Rabbits Treated with Levofloxacin
Mohammed Hayder Asker1*, Hashim H. Al-Zuaini1, Dina Sabah Al-saeed1, Reyam E. Ahmed2, Hamzah Hatem Kareem3
Study design.
a: No expression for KIM-1 (grade 1) in the Medulla of kidney tissue of male rat from control group stained with DAB-brown and counter stained with Mayer heamatoxylin. (100X). b: increased expression of KIM- 1 (black arrows) in the proximal tubules and glomeruli of the EPR for male rat exposed to Levofloxacin. The positive brown expressions were mainly transmembrane, and were located in the colloid material. The immunohistochemical staining was done using DAB as chromogen and counterstained with Mayer heamatoxylin. X100. c: The black arrowheads indicate the Grade-1 expression of KIM-1 in rat kidney tissue with AKI treated with oral Astaxanthin. The significance of few positive expressions was confined to the proximal tubules. There was no immunofluorescence deposition within the glomeruli. Results were done through Immunohistochemical staining by the use of DAB and counter stained with Mayer’s hematoxylin. X400. d: Animals in Combinationgroup (Astaxanthin + Levofloxacin) showing KIM-1 grade 1 (marked by black arrows). The expression was weak and confined to the proximal tubules while none was detected in the glomeruli. Three histostainers were used throughout the immunohistochemical studies: DAB, Mayer’s Hematoxylin and Mayer’s Hematoxylin counterstained DAB. X100.
