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Preliminary Study on Phylogenetic Analysis of Sakub Sheep from Brebes Regency Based on the Mitochondrial DNA (mtDNA) Displacement Loop (D-Loop) Gene Sequence

Preliminary Study on Phylogenetic Analysis of Sakub Sheep from Brebes Regency Based on the Mitochondrial DNA (mtDNA) Displacement Loop (D-Loop) Gene Sequence

Rana Ayuningtyas Adhi Puspita1, Alek Ibrahim2, Endang Tri Margawati3, Ismu Subroto4, Zaenab Nurul Jannah5, Panjono5, Krisna Noli Andrian6, Medania Purwaningrum6, Aris Haryanto6*

1Undergraduate student, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia; 2Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, Indonesia; 3Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, Indonesia; 4Brebes Regency Livestock and Animal Health Service, Brebes, Indonesia; 5Department of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia; 6Department of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

 
*Correspondence | Aris Haryanto, Department of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia; Email: [email protected] 

Figure 1:

Visualization of D-Loop gene amplification results from Sakub sheep samples using 1.5% agarose gel. 1) SKB.1, 2) SKB.2, 3) SKB.3, 4) SKB.4, 5) SKB.5, 6) SKB.6, 7) SKB.7, 8) SKB.8, and M) DNA marker 1kb.

Figure 2:

Position of polymorphic sites in Sakub sheep samples based on D-Loop gene sequence.

Figure 3:

Phylogenetic tree based on D-Loop gene sequence of Sakub sheep samples and reference comparators.

Advances in Animal and Veterinary Sciences

July

Adv. Anim. Vet. Sci., Vol. 13, Iss. 7,

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