Polymorphism in CD40 Gene Associated with the Prevalence of Rheumatoid Arthritis in Pakistan
Polymorphism in CD40 Gene Associated with the Prevalence of Rheumatoid Arthritis in Pakistan
Naila Riaz1*, Muhammad Arshad2 and Faiza Zubair1
ABSTRACT
The polymorphism in CD40 gene have been associated with rheumatoid arthritis (RA). A single SNP rs1535045 was genotyped in 100 RA patients and 100 healthy controls. Some risk factors linked with RA like hypertension, diabetes and smoking were also assessed in the local population of Pakistan. Study subjects included both RA patients and age co-related healthy controls. A total of 100 RA patients with mean age of 51.26 years and individuals for control group were selected in accordance with RA patients’ age. Databases of SNPs provide a powerful resource for association studies that try to establish a relationship between a phenotype and regions of the genome. Total genomic DNA was separated from the blood samples of studied individual. Allele specific PCR based technique was used to study the target SNP for genetic analysis. The SNP, rs1535045 was found to be strongly linked with RA (p<0.01). Genotype and allele counts were assessed by using Chi square analysis. The study suggested that the genotype AT increases the chances of RA by 6.334 times (OR: 6.334, 95% CI: 2.3141–17.333). The risk factors like smoking, diabetes and hypertension were also found to be significantly associated with RA.
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