Molecular Identification and Prevalence of Fasciola gigantica in Cattle and Buffaloes of Punjab, Pakistan
Molecular Identification and Prevalence of Fasciola gigantica in Cattle and Buffaloes of Punjab, Pakistan
Maria Komal1, Kiran Afshan1*, Hafiz Syed Zain Ul Hassan1, Salman Farsi1, Ghulam Narjis2 and Sabika Firasat1
Phylogenetic relationships of partial sequences of the first internal transcribed spacer of the nuclear ribosomal DNA of Fasciola gigantica. The tree with the highest log likelihood (-6673.04) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. All positions containing gaps and missing data were eliminated (complete deletion option).
(A) Inverse relationship between specificity and sensitivity and (B) Direct relationship between 1-specificty and sensitivity. The relationship between frequency and absorbance values (C) shows negative controls absorbance values ranges between 0 and 0.5. (D) and positive controls absorbance values are between 0.5 and 1.5.
(A) The scatter plot of OD values for other helminth parasites, including Fasciola positive and negative controls. (B) The box plot of OD between host sex, age and type. The positive control shows highest OD value, the cutoff point is set at 0.13.
