Molecular Characterization and Genetic Identification of Pakistani Partridges Through DNA-Barcoding
Molecular Characterization and Genetic Identification of Pakistani Partridges Through DNA-Barcoding
Muhammad Zia ul Haq1, Asim Iqbal1, Rana Manzoor Ahmad2, Aftab Ahmad Anjum1, Aqsa Arshad2, Muhammad Wasim1, Sehrish Firyal1, Muhammad Tayyab1, Agha Wasif1 and Ali Raza Awan1*
ABSTRACT
Now a days, DNA barcoding using COI gene (mitochondrial cytochrome c oxidase subunit I) has gained more attention, because of these barcodes validity and simplicity for identification of bird species has increased a lot. In present study, 650bp region of COI gene of two bird species, Chukar patridge (Alectoris chukar) and Sand patridge (Ammoperdix heyi) are sequenced. Total 6 birds (03 birds from each species) were selected. Each sample was aligned with its reference sequence of COI gene available on NCBI and every nucleotide position which did not align with reference sequence was studied to identify SNPs. Our results demonstrated that A. chukar formed a single cluster with Alectoris philbyi while Alectoris rufa and Alectoris melanocephala were a bit distant relatives of A. chukar. The study clearly suggesting efficacy and accuracy of DNA barcodes for molecular identification and characterization of species. Further, barcoding technique used for analysis of molecular diversity and genetic identification of partridges provide a valuable information about population structure, phylogenetic history, molecular conservation and species identification.
To share on other social networks, click on any share button. What are these?
