Impact of Supplementation of Poultry Feed with Locally Characterized Recombinant Thermostable Xylanase on the Growth Performance of Broiler Chicks
Impact of Supplementation of Poultry Feed with Locally Characterized Recombinant Thermostable Xylanase on the Growth Performance of Broiler Chicks
Asma Waris1, Ali Raza Awan1, Sehrish Firyal1, Naeem Rashid2, Abu Seed Hashmi3, Muhammad Wasim1, Shagufta Saeed1 and Muhammad Tayyab1*
ABSTRACT
The current study evaluated the efficiency of locally characterized recombinant thermostable xylanase (XYLTN) from Thermotoga naphthophila in broiler chicks. The XYLTN was produced using BL21 Codon Plus (DE3) cells having pET-21a containing xylanase gene from Thermotoga naphthophila and was used for the supplementation of poultry feed. For the poultry trail, a total of 150 day old broiler chicks were divided into five groups having 30 birds each. Group A served as negative control while groups B, C, and D were experimental groups and fed on a basal diet supplemented with 1000, 1500, and 2000 IU/Kg of locally produced XYLTN, respectively, whereas group E served as positive control and was fed on diet supplemented with 1500 IU/Kg of commercially available xylanase. The supplementation of poultry feed with XYLTN revealed, a maximum weight gain of 1681.25g, feed intake of 2810g, and feed conversion ratio of 1.67 when the feed was supplemented with 2000 IU/Kg of XYLTN. Locally produced XYLTN exhibited promising outcomes compared to positive control which is being utilized currently for the supplementation of poultry feed in the industry. The weight gain, feed intake, and feed conversion ratio of 1681.25g, 2810g, and 1.67 for experimental group D were comparable to 1610.38g, 2830g, and 1.75 for positive control. The ability of enzyme to enhance weight gain, feed consumption, and feed conversion ratio in poultry chicks makes it a strong candidate for replacement of its commercial counterpart being imported for the poultry industry, and its domestic production will contribute to the economic availability of this xylanase for the poultry feed industry.
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