Histo-morphometric Indices and Catalase Response in Muscles, Gills and Intestine of Cadmium Stressed Hypophthalmichthys molitrix
Histo-morphometric Indices and Catalase Response in Muscles, Gills and Intestine of Cadmium Stressed Hypophthalmichthys molitrix
Rida Ajmaal1, Huma Naz1*, Khalid Mehmood1, Moazama Batool2, Najeeb Ur Rehman1, Tanveer Ahmed3*, Syed Qaswar Ali Shah1, Warda Safdar4, Sharjeel Imam1, Muhammad Umar Ijaz5 and Muhammad Usman6
ABSTRACT
The experiment was done to assess at the impact of cadmium (Cd) on histology and antioxidant enzyme (catalase) activity in different tissues (gills, intestine, and muscles) of freshwater fish, Hypophthalmichthys molitrix. For this purpose, acute toxicity of Cd to H. molitrix was determined. Several concentrations (0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5, 30, 32.5, 35, 37.5 and 40 mgL-1) of Cd were given to fish for 96-hr. The LC50 and lethal concentration (96-h) of Cd was found to be 20.661 and 42.801 mgL-1, respectively at 95% confidence interval. Histological examination of gill tissues showed fusion and curling of secondary lamella, degeneration of epithelium of gills and vasodilation in gill filaments. In intestinal tissues, histological alterations including sloughing and degeneration of epithelial cells of intestinal villi were observed. Moreover, significant (p<0.05) increase in height, width and muscularis mucosa of villi and the significant decrease in the crypt depth and tunica mucosa in intestinal tissues of treated fish were exmined. The muscles of fish showed atrophy, muscle fiber’s degeneration and reduction in diameter of muscle fibers were observed. Biochemical analysis showed significant decline (p<0.05) in catalase activity in muscles, intestine, and gills of Cd treated fish. In conclusion, histological and enzymatic changes induced by heavy metals exposure are useful for assessing the harmful impacts of toxicants in various species of fish.
To share on other social networks, click on any share button. What are these?
