Construction, Characterization, and Biosensing Potential of an SOS Inducible mCherry Based Reporter Plasmid
Construction, Characterization, and Biosensing Potential of an SOS Inducible mCherry Based Reporter Plasmid
Shaista Bano1, 2* and Sarfraz A. Tunio2
Fig. 1.
Gel electrophoresis analysis of (A) the amplified mCherry gene of 0.7kb size. (B) pSBM6 with NdeI and HindII enzymes. (C) plasmid map of the resultant construct carrying mCherry under the regulation of SOS inducible promoter.
Fig. 2.
SDS-PAGE analysis of whole cell proteins in the E. coli cells with SOS-inducible mCherry based construct induced with MMC for 2h (A) and uninduced cells (B).
Fig. 3.
Representative fluorescence microscopy images E. coli JM83 containing the SOS inducible mCherry based construct treated with MMC for two hour (A), and uninduced cells (B).
Fig. 4.
A-C: Dose response ratio of fluorescence against ColE7 (A), NA (B) and Nor (C). The experiment was repeated twice in triplicates with similar results and a representative example is shown.
August 2025
Pakistan J. Zool., Vol. 57, Iss. 4, pp. 1503-2002
