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Association of VEGF and IGF2 Polymorphisms with Lambing Number of Small Tail Han Sheep

Association of VEGF and IGF2 Polymorphisms with Lambing Number of Small Tail Han Sheep

Peng Wang1,2, Cai Xia Geng3, Bao Yun Zhang4, Hong Quan Chen3, Ping Qing Wang4, Yu Fang Liu2* and Ming Xing Chu1*

1Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, China
3College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
4Bioengineering College, Chongqing University, Chongqing 400030, China
 
*      Corresponding author: [email protected], [email protected]

Fig. 1.
AvaI-RFLP analysis on amplified region by primer P5 of VEGF in sheep. 
Lanes 1 to 3: genotype CC; lanes 4 to 6: genotype TT; lanes 7 to 9: genotype CT; M: DNA Marker I (TIANGEN, China).
Fig. 2.
PstI-RFLP analysis on amplified region by primer P5 of VEGF in sheep.
Lanes 1 to 4: genotype GT; lanes 5 to 8: genotype GG; M: 20 bp DNA Ladder Marker (TAKARA).
Fig. 3.
BsaHI-RFLP analysis on amplified region by primer P5 of VEGF in sheep.
Lanes 1 and 3: genotype CC; lanes 2, 4, 5 and 6: genotype CT; lanes 7 to 9: genotype TT; M: DNA.
Fig. 4.
SSCP analysis of PCR amplification products of primer P1 in sheep IGF2. 
Lanes 2 and 5: genotype GG; lanes 1,3,6 and 7: genotype GA; lanes 4: genotype AA.
Fig. 5.

Partial sequence comparison of CC and TT genotypes located in the 14752 locus of VEGF in sheep.

Fig. 6.

Partial sequence comparison of GG and GT genotypes at the 14758 locus of sheep VEGF.

Fig. 7.

Partial sequence comparison of CC and TT genotypes at the 14908 locus of sheep VEGF.

Fig. 8.

Sequence comparison of 165 bp of GG and AA genotypes of IGF2 primer P1 in sheep.

Pakistan Journal of Zoology

April

Pakistan J. Zool., Vol. 57, Iss. 2, pp. 501-1001

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