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Association Analysis of Polymorphisms in PLIN1 Gene with the Body Weight of Ross-308 Broiler Chickens

Association Analysis of Polymorphisms in PLIN1 Gene with the Body Weight of Ross-308 Broiler Chickens

Ali A. Abdulkareem1*, Salah H. Faraj2, Mohammed Sabeeh Majeed3, Widya P. B. Putra4, Ratna S. Harahap5

1Department of Animal Production, College of Agriculture and Marshes, University of Thi Qar, Thi Qar, Iraq; 2Department of Biology, College of Science, University of Misan, Maysan, Iraq; 3Al-Manara College for Medical Sciences, Maysan, Iraq; 4Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Cibinong, Bogor, West Java, Indonesia; 5Department of Animal Production, Faculty of Animal Science, University of Jambi, Jambi, Indonesia.

 
*Correspondence | Ali A. Abdulkareem, Department of Animal Production, College of Agriculture and Marshes, University of Thi Qar, Thi Qar, Iraq; Email: [email protected] 

Figure 1:

The primer position (underline) and target sequence of chicken PLIN1 gene (GenBank: NC_052541.1) A fragment 806 bp length with the coding region (grey shading) of exon 5 (1628th - 1749th nucleotide), exon 6 (1961th - 2190th nucleotide) and exon 7 (2264th - 2307th nucleotide).

Figure 2:

The amplification of PLIN1 gene (806 bp) in Ross-308 Broiler chicken on 1% agarose gel. M: DNA ladder 100 bp; Line 1-9: Amplified PCR samples.

Figure 3:

Two novel SNPs in the PLIN1 gene of Ross-308 Broiler chicken i.e. g.1912C>T (A) and g.2308C>T (B).

Advances in Animal and Veterinary Sciences

July

Adv. Anim. Vet. Sci., Vol. 13, Iss. 7,

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