This study was aimed to evaluate the effective methods of alginate extraction from Turbinaria murayana seaweed as a potential feed additive for poultry. The experimental design employed a Completely Randomized Design (CRD) consisting of three extraction methods as treatments, in which each treatment was replicated six times. The procedure of extraction tested included acid, alkaline, and calcium methods. Measured variables were alginate yield, moisture content, ash content, viscosity, molecular weight, and functional group characterization of alginate using Fourier Transform Infrared (FTIR) analysis. The data were statistically analyzed using Analysis of Variance (ANOVA) of CRD and differences among treatments were further assessed using Duncan’s Multiple Range Test (DMRT). The results demonstrated that the extraction methods significantly influenced (p<0.01) the alginate yield, moisture content, ash content, viscosity and molecular weight. The acid extraction method yielded the highest quality alginate, with a yield of 26.93%, moisture content of 13.44%, ash content of 23.74%, viscosity of 15.75 cPs, and a molecular weight of 76,786.97 g/mol. FTIR analysis confirmed that the functional groups of the extracted alginate were consistent with the standard alginate compound. Based on the evaluated parameters, the quality of alginate obtained through the acid extraction method met the criteria established by the Food Chemical Codex 2004.
Keywords | Alginate, Extraction, Feed additive, Poultry, Turbinaria murayana