Multiplex Detection of Citrus Viroids: Advances and Future Application
Multiplex Detection of Citrus Viroids: Advances and Future Application
Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*
ABSTRACT
Two citrus viroids, Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd), have been reported decades ago without further pathological and genetic researches in Taiwan. Here, bioassay, multiplex RT-PCR and multiplex real-time RT-PCR were used to detect the current status of the two viroids showing HSVd was more prevalent than CEVd and simultaneous infections were common in field. Uneven distributions of both viroids also occurred in different tissues of citrus. Two multiplex molecular detection methods provide the understanding of the genetic diversities among viroid isolates and quantify viroids in citrus host. Our field survey can help clarify citrus-viroid relationships and develop proper prevention strategies in the near future.
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