Effect of Alanine on Post-Thaw Quality of Sahiwal Bull Spermatozoa
Effect of Alanine on Post-Thaw Quality of Sahiwal Bull Spermatozoa
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
ABSTRACT
This study was designed to evaluate the effect of alanine on post-thaw quality of Sahiwal bull spermatozoa. Qualifying semen ejaculates [motility (>60%) volume (>4ml) and concentration (>0.5 billion/ml)] collected from three Sahiwal bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled semen was filled in 0.5 ml French straw at 4°C and kept over liquid nitrogen vapors for 10 min. Then straws were plunged into liquid nitrogen (-196°C) for storage. After 24 h of storage, straws were thawed at 37°C for 30 seconds and assessed for sperm motility (%), plasma membrane integrity (%), viability (%), normal apical ridge (%) and chromatin integrity (%). Sperm motility was recorded higher in extender containing 15mM alanine compared to control. Sperm viability was recorded higher in extender containing alanine 10, 15, 20mM compared to control. Sperm plasma membrane integrity, normal apical ridge and chromatin integrity remain similar in all experimental extenders. It is concluded that alanine addition in tris-citric acid extender improved the motility and viability of Sahiwal bull spermatozoa.
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