Xuhuai goat SCD1 was highly conserved and highly expressed in juvenile dorsal longissimus and lactating mammary gland tissue. (A) Phylogenetic relationships of SCD1. Cloned gene sequence was submitted to GenBank with numbers: Homo sapiens, Ovis aris, Capra hircus, Mus musculus, Rattus norvegicus, Suss crofa, and Bos taurus. Phylogenetic relationship of these sequences was estimated by neighbor-joining algorithm using PAM distances. Branch length is proportional to amount of evolution assumed to occur in each lineage. Numbers indicate the percentage of bootstrap replications that support each node. (B) Alignment of goat and mouse SCD1 protein structure, the different amino acid is yellow line. (C) SCD1 is more highly expressed in GOAT mammary gland, liver and lung, but low expressed in muscle. Error bars represent mean value ± SEM. (* p < 0.05, ** p < 0.01). (D) Expression of SCD1 increased significantly during lactation compared with the dry period in mammary gland and lamb during adult period in muscle. Quantitative PCR data were normalized to the dry period. Error bars represent mean value ± SEM. (* p < 0.05, ** p < 0.01).