Agarose gel electrophoresis analysis of one-step RT-PCR amplified products of naturally infected and mechanically inoculated plants. (A) Using CP-RNA2 specific primer, Lane 1: Naturally infected strawberry plants, Lane 2: inoculated Ch. quinoa wild, Lane 3: inoculated Vicia faba, Lane 4: inoculated Fragaria ananassa cv. Festival, Lane 5: inoculated N. tabacum cv. White Burley, Lane 6: empty and M: 100pb Marker (Biomatic-USA). (B) using nepovirus degenerate primers, Lane1: ToRSV as a positive control of group C, Lane 2: naturally infected strawberry plant detection with Nepo-C (s/a) Lane 3: GFLV as a positive control of group A, Lane 4-5: Naturally infected strawberry plant detection with Nepo-A (s/a), Lane 6, 7 and 8: Naturally infected strawberry plant with SLRSV detected with Nepo-A (s/a), Nepo-B (s/a) and Nepo-C (s/a) respectively, Lane 9: Negative control and M: 100pb Marker (Biomatic-USA).