Restriction digestion to confirm the expression constructs. (A) Restriction digestion of pVXC construct to reveal positivity of the clone. CP-PVX full length gene appears at 892 bp while pCDNA vector appears at 5.4 kb, (B) Restriction digestion of pSHX construct to reveal positivity of the clone. shRNA appears at 107 bp while pCDNA vector appears at 5.4 kb.