The results of PCR product 1% Agarose Gel Electrophoresis. Left, The PCR results of 18S rRNA. Right, The PCR results of TFF3. After total RNA reverse transcription and PCR, PCR products by 1% agarose gel electrophoresis, after the completion of the electrophoresis observe in the gel imaging system. The pictures shows the stripes clarity after PCR products by 1% agarose gel electrophoresis, reference gene 18S rRNA amplification, amplified fragment length of 275 bp, consistent with design. TFF3 target gene amplified fragment length of 174 bp, matches the designed length of the primers. Lane 1~4 respectively endotoxin final concentration of 0, 1, 10, 100 μg/mL group, lane 5 is TFF3 induced group, lane 6 is DL-2000 Marker. The results show that the internal gene and TFF3 gene PCR amplification effect is good, achieve the desired effect.