Western blot analysis to detect the internalization of pGH in porcine hepatocytes. Porcine hepatocytes were incubated with FITC-pGH (100 ng/mL) for different durations, and the cytoplasmic and nuclear extracts were subjected and analyzed by Western blotting using 1A3 (the mouse monoclonal antibody to pGH was generated in our laboratory). Anti-β-actin and anti-lamin A antibodies were used as markers for the cytoplasmic and nuclear extracts, respectively. The experiment was performed at least three times.