A, Time kinetics for Biofilm formation. Bacteria were grown for 16 hours and diluted in fresh Luria Bertani medium supplemented with antibiotic. Data was obtained from the average of three independent experiments. B, biofilm formation assays by wild type and fliI complemented Salmonella strains by liquid-interface coverslip assay. C, biofilm formation assays by wild type and fliI complemented Salmonella strains by test tube method. All three strains were grown in LB medium for 96 h. O.D was measured at 595 nm. fliI complemented Salmonella showed best biofilm formation compared to both wild type and fliI deleted SWJ2702. Experiment was performed in duplicate.