PCR amplification of blaIMP and blaVIM genes from E. coli isolates. Ethidium bromide stained agarose gel demonstrating PCR amplification of the blaIMP gene (A) and the blaVIM gene (B) from E. coli isolates. Lane M, base pair size markers; Lane 1, positive control; Lane 2 and 7, negative control; Lanes 3 to 6, blaIMP gene positive samples; Lanes 8 to 11, blaVIM gene positive samples.