(a):Amplified profile of Listeria spp. DNA positive for iap gene at 1500 bp. , Lane(1):Gel Pilot 100 bp ladder , Lane2: positive control, Lane3:Negative control, Lane 4,5,6,7,8,9,10,11 positive isolates. (b): Amplified profile of Listeria monocytogenes. DNA positive for species specific 16S rRNA gene at 1200 bp., Lane(1):Gel Pilot 100 bp ladder, Lane2: positive control, Lane3:Negative control, Lane 4,5,6,7,8,9,10,11positive isolates.