Callus and shoot formation from cotyledon explants following infection with Agrobacterium AGl1 strain harboring NtSAT4 gene; (A) Callus induction of cotyledons after infection with Agrobacterium harboring NtSAT4 gene, growing in MS medium containing BAP 0.75 mgL-1, NAA 0.2 mgL-1 and GA3 0.1 mgL-1 grown at 25 oC under long day conditions. White arrows indicate the callus formation in the cotyledons; (B) Side-view of emerging shoots on shoot initiation medium (MS medium, BAP 3 mgL-1, NAA 0.2 mgL-1 and GA3 0.1 mgL-1 and kanamycin 25 mgL-1); (C) Plants surviving selection in MS media, BAP 0.0125 mgL-1 and kanamycin 50 mgL-1. Scale bar= 3cm.