Relative quantification of CueO mRNA level of K. pneumoniae KW in response to Cu+2. A, bacterial cultures (20 ml) were grown and Cu+2 stress ranging 0 to 5mM was given at early log phase stage. Total RNA isolated was subjected to RT PCR. cDNA synthesized were used for real time PCR. RNA level in each sample normalized to GyrA (housekeeping gene) were quantified in relation to that in control (0mM Cu+2). A gradual increase in CueO mRNA was observed up to 4 mM Cu+2 that decreased with further increase in medium Cu+2; B, RNA level was measured with respect to Cu+2 exposure time in the presence of 4 mM Cu+2. A bimodal increase in CueO mRNA level was found with two maxima, first at 15 and 30 min and second at 90 min.