Fig. 2.
Electrophoretic patterns of SNP genotyping by PCR-RFLP method; (a) is the PCR-HinfI-RFLP analysis result of SNP at g.1500G>A in intron 2 of the CFL2 gene, genotype GA (389 + 279 + 110 bp), genotype AA (279 + 110 bp) and genotype GG (389 bp); (b) is the PCR-VspI-RFLP analysis result of SNP at g.1694T>A in exon 4 of the CFL2 gene, genotype AT (197 + 172 + 25 bp, 25 bp fragment was too small to stay in gel), genotype TT (172 + 25 bp) and genotype AA (197 bp); (c) is the PCR- HaeIII-RFLP analysis result of SNP at g.2213C>G in 3’UTR of the CFL2 gene, genotype CG (276 + 252 + 24 bp; 24 bp fragment was too small to stay in gel), genotype CC (252 + 24 bp) and genotype GG (276 bp); M refers to the DNA molecular weight marker.