FSH and LH levels in the serum and mRNA expression in pituitary. A, serum samples were collected from five groups during the treatment phase (D2, D6, D10, D14, D18) and once before medication as a baseline value. All sampling times were during the metestrus stage of each cycle. Mifepristone downregulated the level of FSH. However, following treatment with Bu Shen Zhu Yun formula (BSZYF), FSH levels ìncreased to the level of the blank group. There were no significant differences in LH levels between the groups. The levels of FSH and LH in the serum were measured by ELISA; B, differences in FSHβ and LHβ mRNA expression in the anterior pituitary tissue were similar to differences in serum levels. Values are represented as mean ± SD (n=6 per group), *P<0.05 compared with the blank group, #P<0.05 compared with the model group.