Effect of BHS-1 on proliferation and cell death of A549 and A549/B cells. A, Chemical structure of BHS-1; B, A549 and A549/B cells were treated with indicated concentrations of BHS-1 for 24 h and cell proliferation was determined by MTT assay. Data are expressed as Mean±SEM of 3 different experiments. Columns not sharing the same superscript letter differ significantly (P<0.05); C, A549 and A549/B cells were treated with 30 μM BHS-1 for 24 h and cell death was evaluated by live/dead assay; D, Data from live/dead assay are expressed as Mean±SEM of 3 different experiments. Columns not sharing the same superscript letter differ significantly (P<0.05).