A, Analysis of IFNα2-Tα1gene by 1% agarose gel electrophoresis (Lane M, DNA ladder; Lane 1, IFNα2-Tα1gene (591 bp)). B, Analysis of recombinant E. coli BL21 (DE3) clones by colony PCR(Lane M, DNA ladder; Lane 1-7, recombinant clones). C, Analysis of SUMO- IFNα2-Tα1 fusion protein by 12 % SDS-PAGE (Lane M, protein ladder; Lane 1, un-induced cell fraction; Lane 2, total cell lysate; Lane 3, insoluble cell pellet; Lane 4, soluble cell fraction).