Brucella genus-specific PCR results of the strains isolated from five reference strains and two isolates from two pet dogs. Lane M, molecular weight marker; Lane 1, negative control with no DNA added; Lane 2, positive control with DNA extracted from the B. melitensis strain (16M); Lane 3, DNA extracted from isolate B. abortus (544A); Lane 4, DNA extracted from the B. suis (S2); Lane 5, DNA extracted from isolate strain W5; Lane 6, DNA extracted from isolate strain Y4.