Purification and immuno blot analysis. A, Purification of recombinant SUMO-IFNα2-Tα1 fusion protein by Ni+ affinity chromatography (Lane M, Protein marker; Lane 1, Purified SUMO-IFNα2-Tα1). B, Analysis of SUMO-IFNα2-Tα1 cleavage reaction with SUMO protease by 12 % SDS-PAGE (Lane M, Protein marker; Lane 1, FNα2-Tα1 and SUMO bands after digestion). C, Purification of FNα2-Tα1 by Ni+ affinity chromatography from cleavage reaction (Lane M, Protein marker; Lane 1, purified FNα2-Tα1). D, Immuno blot analysis with anti-interferon α2 antibody. E, Immuno blot analysis with anti-thymosin α-1 antibody.