Sensitivity tests for real-time PCR and DARPM. Serial 10-fold dilutions of CPV DNA and CPV virus culture supernatant (CPV DNA: 1.39×108 copies·μL-1 to 1.39×100 copies·μL-1, CPV virus culture supernatant: 7.11×108 copies·μL-1 to 7.11×100 copies·μL-1) were detected. A minimum of 1.53×101 copies of CPV DNA and 6.70×101 copies of CPV virus culture supernatant were detected. A: 1–9: Equal dilutions of CPV DNA at 10-1–10-9; B: 1–9: CPV virus culture supernatant at 10-1–10-9.