Phylogenetic tree of partial VP2 nucleotide sequences of canine parvovirus strains obtained from the GenBank database and Pakistani CPV strains. The following sequences were added in the GenBank database: CPV isolate 435/USA (AY742953.1), CPV isolate 431/USA(Y742951.1), CPV2aisolate/Henan32/China(KM924289.1),CPV2aisolate/geneHeN02/08/China(EU441280.1), CPV isolate BJ1/2010/ China: Beijing/ VP2 gene, CPV 2a isolate ME40_ECU2012/Ecuador(KF149983.1), CPV 2a isolate ME39_ECU2012/Ecuador(KF149982.1), CPV isolate/Japan (AB128923.1), CPV isolate vac6/india (JN625224.1),CPV isolate vac1/india (JN625219.1), CPV isloate 271/12-Miky/italy (JX305965.1), CPV 503/11-5/Italy(JX305964.1), CPV isloate 480/10/Italy(JX305957.1), CPV 2c isolate 219/08-13 (FJ005250.1), CPV isolate-U51/USA(AY742942.1), and CPV-632 isolate/USA(AY742940.1).