Figure 3:

SSF of wheat straw by adapted ethanol and lactic acid fermenting strains. (a) Ethanol fermentation using freshly pretreated rice straw from S. cerevisiae Angel. Conditions: 20 % w/w solids loading, 15 FPU/g DM cellulase dosage (youtell#7 purchased enzyme) and onsite cellulase with same nutrients composition of SM except glucose. Prehydrolysis was performed for 6 h at 50 °C, pH 4.8, and 150 rpm. Solid seeds culture was inoculated in SSF system. Real SSF was performed for 72 h at 37 °C, pH 4.8, 150 rpm, (b) Lactic acid fermentation using freshly pretreated rice straw from Pediococcus sp. Conditions: 20 % w/w solids loading, 15 FPU/g DM cellulase dosage (onsite produced cellulase) with same nutrients composition of MRS except glucose. Prehydrolysis was performed for 6 h at 50°C, pH 4.8, and 150 rpm. Real SSF was performed for 72 h at 42 °C, pH 5.5, 150 rpm, (c). Lactic acid fermentation from Lactobacillus sp. other conditions were as mentioned for b., (d). Lactic acid fermentation using adapted B. coagulans other conditions were same as mentioned for b.