Number of step 7 spermatids following 10ρg (A), 1ηg (B) and 1µg (C) doses after 2, 4, 6, 8, 10 and 12 days of alone kisspeptin treatment. Step 7 spermatids decreased significantly in 1ηg and 1µg treatment groups after 4 days of treatment and thereafter, decreased gradually up to 12 days. 10ρg dose of kisspeptin caused a decrease in step 7 spermatids only after 12 days of treatment. Values are expressed as mean ± SE (n=7). *p < 0.05, **p < 0.01, ***p< 0.001.