Roles of FGF10 on distinct hypothalamic axons. Vasopressin (A) and TH (B) positive neurons are present within the E4 explants. After immunolabelling with Tuj1, axons extend towards FGF10 soaked beads, but one axon (TH+) turn (arrow), rather than project into FGF10 beads (C). Vasopressin axon goes directly towards FGF10 sources (arrow, D); while TH axon turns away from FGF10 beads (arrow, E). Scale bars represent 50μm.