Growth curves of Desulfovibrio spp. A, Desulfovibrio spp. was grown in lactate sulfate broth for 7 days at pH 7, incubated at 37oC. O.D was determined at 590 nm. Maximum growth was observed after 3 days. B, Effect of temperature: Desulfovibrio spp. was grown in lactate sulfate broth for 72 h at different temperatures (25-45oC). O.D was determined at 590 nm. Highest growth was observed at 37oC. C, Effect of pH: Desulfovibrio spp. was grown in lactate sulfate broth for 72 hours at different pH, incubated at 37oC. O.D was determined at 590 nm. Maximum growth was observed at pH 7. Error bars in figures represent standard deviation (n=3).