Assessing the specificity of RT-LAMP for PRRSV. Samples were isolated from pigs infected with PRRSV, CSFV, PCV-2, PPV, and PRV and tested usig the RT-LAMP assay. The output of the assay was visualized using (A) agarose gel electrophoresis and (C) color change with SYBR Green I dye. (B) The presence of PRRSV in the samples was confirmed by RT-PCR and visualized using agarose gel electrophoresis. Lanes: M; DL2000 Marker (A and B only). 1; CSFV, 2; PCV-2, 3; PPV, 4; PRRSV, 5; PRV.