Assessing the sensitivity of the PRRSV RT-LAMP assay. 10-fold serial dilutions of a stock PRRSV RNA template (10ng/μL) were used to assess the sensitivity of the RT-LAMP assay compared to RT-PCR. Output from the RT-LAMP assay was visualized by (A) agarose gel electrophoresis and (C) color change with SYBR Green I dye. (B) RT-PCR amplification of PRRSV RNA was visualized by agarose gel electrophoresis. Lanes: M; DL2000 Marker (A and B only); 1-5: 10-1, 10-2, 10-3, 10-4, and 10-5 dilutions of the stocktemplate.