Agarose gel electropherograms of the fliI from wild type and fliI complemented Salmonella using phusion PCR. 1st Lane, 1kb DNA ladder plus PageRulerTM Prestained Protein Ladder; 2nd Lane, fliI (wild type); 3rd – 5th Lane, fliI from fliI complemented Salmonella at different annealing temperatures of 60, 62 and 65°C.