Agarose gel electrophoresis of multiplex PCR of pfhA (275 bp), hgbB (499 bp), tbpA (728 bp), and toxA (846 bp) virulence genes for characterization of Pasteurella multocida.
Lane M: 100 bp ladder as a molecular size DNA marker. Lane C+: Control positive for pfhA, hgbB, tbpA and toxA genes. Lane C-: Control negative. Lanes 1, 3, 4, 5, 7 & 10: Positive strains for hgbB gene. Lanes 2, 8 & 9: Positive strains for pfhA gene. Lane 2: Positive strain for tbpA gene.
Lane 10: Positive strain for toxA gene. Lane 6: Negative strain for pfhA, hgbB, tbpA and toxA genes.