Untreated MCF-7 cell (a), arrow-nuclear membrane, circle-cell membrane, arrow head-compact mitochondrion. MCF-7 cell treated with IC50 value of silymarin (b), Arrow-secondary lysosomes, Arrow head-loss of cristae, two headed arrow-granulated cytoplasm, asterisk-cleavage of nuclear membrane (formation of pyknotic nucleus). Silymarin-loaded SLN treated MCF-7 cell (c), arrow-extreme chromatin condensation, rectangle-disintegrated cell membrane and asterisk-fragmentation of the cytoskeleton (huge holes on cytoskeleton).